Multilocus Microsatellite Typing reveals intra-focal genetic diversity among strains of Leishmania tropica in Chichaoua Province, Morocco

被引:14
作者
Krayter, Lena [1 ]
Alam, Mohammad Zahangir [1 ]
Rhajaoui, Mohamed [2 ]
Schnur, Lionel F. [3 ]
Schoenian, Gabriele [1 ]
机构
[1] Charite, Inst Microbiol & Hyg, D-12203 Berlin, Germany
[2] Natl Inst Hyg, Dept Parasitol, Rabat, Morocco
[3] Hebrew Univ Jerusalem, Hadassah Med Sch, Kuvin Ctr Study Infect & Trop Dis, Dept Parasitol, IL-91010 Jerusalem, Israel
关键词
Leishmania tropica; Morocco; MLMT; Intra-focal diversity; Chichaoua; Marrakech; CANINE VISCERAL LEISHMANIASIS; CUTANEOUS LEISHMANIASIS; CAUSATIVE AGENT; OLD-WORLD; IDENTIFICATION; HETEROGENEITY; FOCUS;
D O I
10.1016/j.meegid.2014.09.037
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In Morocco, cutaneous leishmaniasis (CL) caused by Leishmania (L.) tropica is a major public health threat. Strains of this species have been shown to display considerable serological, biochemical, molecular biological and genetic heterogeneity; and Multilocus Enzyme Electrophoresis (MLEE), has shown that in many countries including Morocco heterogenic variants of L. tropica can co-exist in single geographical foci. Here, the microsatellite profiles discerned by MLMT of nine Moroccan strains of L. tropica isolated in 2000 from human cases of CL from Chichaoua Province were compared to those of nine Moroccan strains of L. tropica isolated between 1988 and 1990 from human cases of CL from Marrakech Province, and also to those of 147 strains of L. tropica isolated at different times from different worldwide geographical locations within the range of distribution of the species. Several programs, each employing a different algorithm, were used for population genetic analysis. The strains from each of the two Moroccan foci separated into two phylogenetic clusters independent of their geographical origin. Genetic diversity and heterogeneity existed in both foci, which are geographically close to each other. This intra-focal distribution of genetic variants of L. tropica is not considered owing to in situ mutation. Rather, it is proposed to be explained by the importation of pre-existing variants of L. tropica into Morocco. (C) 2014 The Authors. Published by Elsevier B.V.
引用
收藏
页码:233 / 239
页数:7
相关论文
共 33 条
[1]   Canine Leishmania infantum enzymatic polymorphism: A review including 1023 strains of the Mediterranean area, with special reference to Algeria [J].
Ait-Oudhia, K. ;
Harrat, Z. ;
Benikhlef, R. ;
Dedet, J. P. ;
Pratlong, F. .
ACTA TROPICA, 2011, 118 (02) :80-86
[2]   Demonstration of Genetic Exchange During Cyclical Development of Leishmania in the Sand Fly Vector [J].
Akopyants, Natalia S. ;
Kimblin, Nicola ;
Secundino, Nagila ;
Patrick, Rachel ;
Peters, Nathan ;
Lawyer, Phillip ;
Dobson, Deborah E. ;
Beverley, Stephen M. ;
Sacks, David L. .
SCIENCE, 2009, 324 (5924) :265-268
[3]   Leishmaniasis Worldwide and Global Estimates of Its Incidence [J].
Alvar, Jorge ;
Velez, Ivan D. ;
Bern, Caryn ;
Herrero, Merce ;
Desjeux, Philippe ;
Cano, Jorge ;
Jannin, Jean ;
den Boer, Margriet .
PLOS ONE, 2012, 7 (05)
[4]   Moroccan Leishmania infantum: Genetic Diversity and Population Structure as Revealed by Multi-Locus Microsatellite Typing [J].
Amro, Ahmad ;
Hamdi, Salsabil ;
Lemrani, Meryem ;
Mouna, Idrissi ;
Mohammed, Hida ;
Mostafa, Sabri ;
Rhajaoui, Mohamed ;
Hamarsheh, Omar ;
Schoenian, Gabriele .
PLOS ONE, 2013, 8 (10)
[5]  
[Anonymous], 1996, GENETIX 4 05 LOGICIE
[6]   PHYLOGENETIC ANALYSIS - MODELS AND ESTIMATION PROCEDURES [J].
CAVALLISFORZA, LL ;
EDWARDS, AWF .
EVOLUTION, 1967, 21 (03) :550-+
[7]   LEISHMANIA-TROPICA IN MOROCCO - INFECTION IN DOGS [J].
DEREURE, J ;
RIOUX, JA ;
GALLEGO, M ;
PERIERES, J ;
PRATLONG, F ;
MAHJOUR, J ;
SADDIKI, H .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1991, 85 (05) :595-595
[8]   MICROSATELLITE ANALYSER (MSA): a platform independent analysis tool for large microsatellite data sets [J].
Dieringer, D ;
Schlotterer, C .
MOLECULAR ECOLOGY NOTES, 2003, 3 (01) :167-169
[9]   Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study [J].
Evanno, G ;
Regnaut, S ;
Goudet, J .
MOLECULAR ECOLOGY, 2005, 14 (08) :2611-2620
[10]   CONVERT: A user-friendly program to reformat diploid genotypic data for commonly used population genetic software packages [J].
Glaubitz, JC .
MOLECULAR ECOLOGY NOTES, 2004, 4 (02) :309-310