Protective effect of three diallyl sulphides against glucose-induced erythrocyte and platelet oxidation, and ADP-induced platelet aggregation

Isolated human erythrocyte membrane and platelet, were used to study the antioxidative and anti-aggregative effects of three diallyl sulphides (diallyl sulphide, DAS; diallyl disulphide, DADS; diallyl trisulphide, DAT) against glucose-induced oxidation and adenosine 5'diphosphate (ADP)-induced platelet aggregation. Three sulphide agents showed dose-dependent antioxidative protection against glucoseinduced enythrocyte membrane oxidation (p<.05), and these agents at 10 muM significantly increased the retention of alpha-tocopherol in erythrocyte membrane (p <.05), in which DAT was the most effective agent (p <.05). Three sulphide agents significantly inhibited 30 and 50 mM of glucose-induced platelet oxidation (p <.05). The anti-aggregative activity of each sulphide agent was dose dependent (p <.05), in which DAT showed the greatest inhibitory effect on platelet aggregation than DADS, followed by DAS (p <.05). After ADP stimulation, the malondialdehyde (MDA) formation in platelets treated with sulphide agents was significantly less (p<.05), in which DADS and DAT showed similar antioxidative activities (p>.05). These results suggested that DADS and DAT could be considered as strong antioxidative and antithrombotic agents to prevent or control oxidative damage and platelet hyperactivity. (C) 2003 Elsevier Science Ltd. All rights reserved.