Point mutations in the squalene epoxidase gene of Indian ITS genotype VIII T. mentagrophytes identified after DNA isolation from infected scales

被引：41

作者：

Burmester, Anke ^{[1
]}

Hipler, Uta-Christina ^{[1
]}

Hensche, Reka ^{[1
]}

Elsner, Peter ^{[1
]}

Wiegand, Cornelia ^{[1
]}

机构：

[1] Univ Hosp Jena, Dept Dermatol, Erfurter Str 35, D-07743 Jena, Thuringia, Germany

来源：

MEDICAL MYCOLOGY CASE REPORTS | 2019年 / 26卷

关键词：

Terbinafine resistance; Squalene epoxidase; Trichophyton mentagrophytes; Indian ITS genotype VIII; STRAINS;

D O I：

10.1016/j.mmcr.2019.09.001

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Terbinafine resistant Indian ITS genotype VIII T. mentagrophytes strain was identified by partial sequencing of the squalene epoxidase gene using DNA isolated from infected scales. This method allowed the rapid identification of single point mutations within the squalene epoxidase gene, even before a fungal culture was obtained. Terbinafine resistance was indicated by the amino acid position switch Phe397Leu based on a single point mutation of the codon changing it from TTC to CTC.

引用

页码：23 / 24

页数：2

共 7 条

[1]

de Hoog GS, 1998, MYCOSES, V41, P183, DOI 10.1111/j.1439-0507.1998.tb00321.x

[2] Signature polymorphisms in the internal transcribed spacer region relevant for the differentiation of zoophilic and anthropophilic strains of Trichophyton interdigitale and other species of T. mentagrophytes sensu lato [J].

Heidemann, S. ;

Monod, M. ;

Graeser, Y. .

BRITISH JOURNAL OF DERMATOLOGY, 2010, 162 (02) :282-295

[3] The current Indian epidemic of superficial dermatophytosis due to Trichophyton mentagrophytes-A molecular study [J].

Nenoff, Pietro ;

Verma, Shyam B. ;

Vasani, Resham ;

Burmester, Anke ;

Hipler, Uta-Christina ;

Wittig, Franziska ;

Krueger, Constanze ;

Nenoff, Kolja ;

Wiegand, Cornelia ;

Saraswat, Abir ;

Madhu, Rengarajan ;

Panda, Saumya ;

Das, Anupam ;

Kura, Mahendra ;

Jain, Akshay ;

Koch, Daniela ;

Graeser, Yvonne ;

Uhrlass, Silke .

MYCOSES, 2019, 62 (04) :336-356

[4] A clarion call for preventing taxonomical errors of dermatophytes using the example of the novel Trichophyton mentagrophytes genotype VIII uniformly isolated in the Indian epidemic of superficial dermatophytosis [J].

Nenoff, Pietro ;

Verma, Shyam B. ;

Uhrlass, Silke ;

Burmester, Anke ;

Graeser, Yvonne .

MYCOSES, 2019, 62 (01) :6-10

[5]

Rudramurthy SM, 2018, ANTIMICROB AGENTS CH, V62, DOI [10.1128/aac.02522-17, 10.1128/AAC.02522-17]

[6] High terbinafine resistance in Trichophyton interdigitale isolates in Delhi, India harbouring mutations in the squalene epoxidase gene [J].

Singh, Ashutosh ;

Masih, Aradhana ;

Khurana, Ananta ;

Singh, Pradeep Kumar ;

Gupta, Meenakshi ;

Hagen, Ferry ;

Meis, Jacques F. ;

Chowdhary, Anuradha .

MYCOSES, 2018, 61 (07) :477-484

[7]

Yamada T, 2017, ANTIMICROB AGENTS CH, V61, DOI [10.1128/AAC.00115-17, 10.1128/aac.00115-17]

← 1 →