Immune rejection of human dystrophin following intramuscular injections of naked DNA in mdx mice

被引：45

作者：

Braun, S ^{[1
]}

Thioudellet, C ^{[1
]}

Rodriguez, P ^{[1
]}

Ali-Hadji, D ^{[1
]}

Perraud, F ^{[1
]}

Accart, N ^{[1
]}

Balloul, JM ^{[1
]}

Halluard, C ^{[1
]}

Acres, B ^{[1
]}

Cavallini, B ^{[1
]}

Pavirani, A ^{[1
]}

机构：

[1] Transgene SA, F-67082 Strasbourg, France

来源：

GENE THERAPY | 2000年 / 7卷 / 17期

关键词：

dystrophin; mdx; immunology; Duchenne; plasmid; gene therapy;

D O I：

10.1038/sj.gt.3301261

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intramuscular administration of plasmid expressing full-length human dystrophin in dystrophin-deficient adult mdx mice resulted in humoral and weak specific T cell responses against the human dystrophin protein. Following plasmid injection, human dystrophin was detected in the injected muscles at 7 days, but decreased thereafter Anti-dystrophin antibodies were found 21 days following plasmid injection, which coincided with transient myositis. This immune rejection prevented the mice from expressing human dystrophin after a second plasmid injection. No anti-DNA antibodies were found. Anti-dystrophin antibodies were seen in a smaller proportion of plasmid-injected dystrophin-competent C57BL/10 mice, suggesting that the immune rejection of dystrophin may be explained partially by species differences in the dystrophin protein.

引用

页码：1447 / 1457

页数：11

共 70 条

[1] HUMAN DYSTROPHIN EXPRESSION IN MDX MICE AFTER INTRAMUSCULAR INJECTION OF DNA CONSTRUCTS [J].