Simply and sensitively simultaneous detection hepatocellular carcinoma markers AFP and miRNA-122 by a label-free resonance light scattering sensor

被引：45

作者：

Chen, Feng ^{[1
]}

Zhang, Feng ^{[2
]}

Liu, Yi ^{[1
]}

Cai, Changqun ^{[1
]}

机构：

[1] Xiangtan Univ, Coll Chem, Key Lab Green Organ Synth & Applicat Hunan Prov, Key Lab Environm Friendly Chem & Applicat,Minist, Xiangtan 411105, Peoples R China

[2] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China

来源：

TALANTA | 2018年 / 186卷

基金：

中国国家自然科学基金;

关键词：

Simultaneous detection; AFP; miRNA; Bifunctional; Label-free; ALPHA-FETOPROTEIN; TUMOR-MARKERS; ELECTROCHEMICAL IMMUNOSENSOR; CANCER; IMMUNOASSAY; NANOCOMPOSITES; FLUORESCENCE; BIOMARKERS; MICRORNAS;

D O I：

10.1016/j.talanta.2018.04.060

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, an intelligent and label-free sensor is utilized for the first time to one-spot simultaneous detection hepatocellular carcinoma markers AFP and miRNA-122 by a resonance light scattering (RLS) sensor. cDNA1 hybridizes with cDNA2 to form double-stranded DNA (dsDNA). The construction of dsDNA and methyl violet is used to form the RLS sensor via the electronic interaction. When AFP or miRNA-122 is present, the cDNA (cDNA1 or cDNA2) can bindings of target, thereby RLS intensity changed proportionally with the concentration of AFP or that of miRNA-122. The detection limits of AFP and miRNA-122 are 0.94 mu g/L and 98 pM respectively, and their good linear which ranges from 5 to 100 mu g/L and 200 pM to 10 nM are achieved using the assay. In the presence of miRNA-122 and AFP mixtures, AFP bound to the AFP aptamer to increase the RLS signal, and miRNA-122 bound to the miRNA-122 complementary strand to decrease the RLS signal. The RLS signal changed in response to changing AFP and miRNA-122 concentrations, so that one-spot simultaneous detection of alpha fetal protein and miRNA-122 is achieved. This method has potential practical applications in the research of hepatocellular carcinoma.

引用

页码：473 / 480

页数：8

共 34 条

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