Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify MLST clade 4 Clostridium difficile isolates

被引：12
作者：
Cheng, Jing-Wei [1 ,2 ]
Liu, Chang [1 ,2 ]
Kudinha, Timothy [3 ,4 ]
Xiao, Meng [1 ]
Yu, Shu-Ying [1 ,2 ]
Yang, Chun-Xia [5 ]
Wei, Ming [5 ]
Liang, Guo-Wei [6 ]
Shao, Dong-Hua [6 ]
Kong, Fanrong [3 ]
Tong, Zhao-Hui [7 ]
Xu, Ying-Chun [1 ]
机构：
[1] Chinese Acad Med Sci, Dept Clin Lab, Peking Union Med Coll Hosp, Beijing, Peoples R China
[2] Chinese Acad Med Sci, Grad Sch, Peking Union Med Coll, Beijing, Peoples R China
[3] Charles Sturt Univ, Orange, NSW, Australia
[4] Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW, Australia
[5] Capital Med Univ, Beijing Chao Yang Hosp, Dept Infect Dis & Clin Microbiol, Beijing, Peoples R China
[6] Aerosp Ctr Hosp, Dept Clin Lab, Beijing, Peoples R China
[7] Capital Med Univ, Beijing Chao Yang Hosp, Dept Resp & Crit Care Med, Beijing, Peoples R China
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 92卷 / 01期
关键词：
Clostridium difficile; Typing; MLST; Clade; 4; MALDI-TOF MS; ANTIMICROBIAL SUSCEPTIBILITY; EPIDEMIOLOGY; IDENTIFICATION; RESISTANCE; INFECTION;
D O I：
10.1016/j.diagmicrobio.2018.04.011
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Clostridium difficile is the leading cause of health care-associated infections. Previous studies suggest that C. difficile MLST clade 4 strains with higher drug resistance rates constitute the major clone spreading in China. Thus development of a rapid and accurate typing method for these strains is needed to monitor the epidemiology of this clone and to guide clinical treatment A total of 160 non-duplicate C. difficile isolates recovered from three large teaching hospitals in Beijing were studied. All the 41 clade 4 C. difficile isolates clustered together on the PCA dendrogram. Spectra peak statistics revealed that five markers (2691.43Da, 2704.91Da, 2711.93Da, 3247.27Da and 3290.76Da) can easily and reliably distinguish between clade 4 and non-clade 4 isolates, with area under the curve (AUC) values of 0.991, 0.997, 0.973,1 and 1, respectively. In conclusion, MALDI-TOF MS is a very simple and accurate method for identifying C. difficile MLST clade 4 strains. (C) 2018 Elsevier Inc. All rights reserved.
引用
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页码：19 / 24
页数：6
相关论文
共 24 条
  • [1] Bassetti M, 2012, EXPERT REV ANTI-INFE, V10, P1405, DOI [10.1586/ERI.12.135, 10.1586/eri.12.135]
  • [2] Comparative Genome Analysis and Global Phylogeny of the Toxin Variant Clostridium difficile PCR Ribotype 017 Reveals the Evolution of Two Independent Sublineages
    Cairns, M. D.
    Preston, M. D.
    Hall, C. L.
    Gerding, D. N.
    Hawkey, P. M.
    Kato, H.
    Kim, H.
    Kuijper, E. J.
    Lawley, T. D.
    Pituch, H.
    Reid, S.
    Kullin, B.
    Riley, T. V.
    Solomon, K.
    Tsai, P. J.
    Weese, J. S.
    Stabler, R. A.
    Wren, B. W.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (03) : 865 - 876
  • [3] Molecular Epidemiology and Antimicrobial Susceptibility of Clostridium difficile Isolates from a University Teaching Hospital in China
    Cheng, Jing-Wei
    Xiao, Meng
    Kudinha, Timothy
    Kong, Fanrong
    Xu, Zhi-Peng
    Sun, Lin-Ying
    Zhang, Li
    Fan, Xin
    Xie, Xiu-Li
    Xu, Ying-Chun
    [J]. FRONTIERS IN MICROBIOLOGY, 2016, 7
  • [4] The Role of Glutamate Dehydrogenase (GDH) Testing Assay in the Diagnosis of Clostridium difficile Infections: A High Sensitive Screening Test and an Essential Step in the Proposed Laboratory Diagnosis Workflow for Developing Countries like China
    Cheng, Jing-Wei
    Xiao, Meng
    Kudinha, Timothy
    Xu, Zhi-Peng
    Sun, Lin-Ying
    Hou, Xin
    Zhang, Li
    Fan, Xin
    Kong, Fanrong
    Xu, Ying-Chun
    [J]. PLOS ONE, 2015, 10 (12):
  • [5] Structural characterization of CYP144A1-a cytochrome P450 enzyme expressed from alternative transcripts in Mycobacterium tuberculosis
    Chenge, Jude
    Kavanagh, Madeline E.
    Driscoll, Max D.
    McLean, Kirsty J.
    Young, Douglas B.
    Cortes, Teresa
    Matak-Vinkovic, Dijana
    Levy, Colin W.
    Rigby, Stephen E. J.
    Leys, David
    Abell, Chris
    Munro, Andrew W.
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [6] Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology
    Clark, Andrew E.
    Kaleta, Erin J.
    Arora, Amit
    Wolk, Donna M.
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2013, 26 (03) : 547 - 603
  • [7] Clinical and Laboratory Standards Institute (CLSI), 2017, METH ID CULT MICR US
  • [8] Antimicrobial susceptibility and resistance mechanisms of clinical Clostridium difficile from a Chinese tertiary hospital
    Dong, Danfeng
    Zhang, Lihua
    Chen, Xu
    Jiang, Cen
    Yu, Beiqin
    Wang, Xuefeng
    Peng, Yibing
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 41 (01) : 80 - 84
  • [9] Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization-Tandem Time of Flight Mass Spectrometry
    Fagerquist, Clifton K.
    Zaragoza, William J.
    Sultan, Omar
    Woo, Nathan
    Quinones, Beatriz
    Cooley, Michael B.
    Mandrell, Robert E.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (09) : 2928 - 2940
  • [10] Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak
    Griffin, Paul M.
    Price, Gareth R.
    Schooneveldt, Jacqueline M.
    Schlebusch, Sanmarie
    Tilse, Martyn H.
    Urbanski, Tess
    Hamilton, Brett
    Venter, Deon
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (09) : 2918 - 2931
123