MALDI-MSI as a Complementary Diagnostic Tool in Cytopathology: A Pilot Study for the Characterization of Thyroid Nodules

被引：23

作者：

Capitoli, Giulia ^{[1
]}

Piga, Isabella ^{[2
]}

Galimberti, Stefania ^{[1
]}

Leni, Davide ^{[3
]}

Pincelli, Angela Ida ^{[4
]}

Garancini, Mattia ^{[5
]}

Clerici, Francesca ^{[2
]}

Mahajneh, Allia ^{[2
]}

Brambilla, Virginia ^{[6
]}

Smith, Andrew ^{[2
]}

Magni, Fulvio ^{[2
]}

Pagni, Fabio ^{[6
]}

机构：

[1] Univ Milano Bicocca, Dept Med & Surg, Ctr Biostat Clin Epidemiol, I-20900 Vedano Al Lambro, Italy

[2] Univ Milano Bicocca, Dept Med & Surg, Prote & Metabol Platform, I-20900 Vedano Al Lambro, Italy

[3] San Gerardo Hosp, Dept Radiol, I-20900 Asst Monza, Italy

[4] San Gerardo Hosp, Dept Endocrinol, I-20900 Asst Monza, Italy

[5] San Gerardo Hosp, Dept Surg, I-20900 Asst Monza, Italy

[6] Univ Milano Bicocca, San Gerardo Hosp, Dept Med & Surg, Pathol, I-20900 Asst Monza, Italy

来源：

CANCERS | 2019年 / 11卷 / 09期

关键词：

thyroid carcinoma; MALDI-MSI; proteomics; Fine Needle Aspiration; PROTEOMICS; CANCER;

D O I：

10.3390/cancers11091377

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The present study applies for the first time as Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Imaging (MSI) on real thyroid Fine Needle Aspirations (FNAs) to test its possible complementary role in routine cytology in the diagnosis of thyroid nodules. The primary aim is to evaluate the potential employment of MALDI-MSI in cytopathology, using challenging samples such as needle washes. Firstly, we designed a statistical model based on the analysis of Regions of Interest (ROIs), according to the morphological triage performed by the pathologist. Successively, the capability of the model to predict the classification of the FNAs was validated in a different group of patients on ROI and pixel-by-pixel approach. Results are very promising and highlight the possibility to introduce MALDI-MSI as a complementary tool for the diagnostic characterization of thyroid nodules.

引用

页数：11

共 29 条

[1] Selective profiling of proteins in lung cancer cells from fine-needle aspirates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry [J].