A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus

被引：2

作者：

Wang, Yumei ^{[1
]}

Liu, Yan ^{[1
]}

Ding, Yaping ^{[2
]}

Sun, Nan ^{[1
]}

Gong, Yafang ^{[3
]}

Gao, Shangxian ^{[1
]}

机构：

[1] Natl Inst Food & Drug Control, Dept In Vitro Diagnost Reagents, Key Lab, Minist Hlth Res Qual & Standardizat Biotech Prod, Beijing 100050, Peoples R China

[2] Shenzhen Kangmei Biotechnol Co Ltd, Shenzhen 518057, Peoples R China

[3] Cent S Univ, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2014年 / 159卷 / 12期

关键词：

CERVICAL INTRAEPITHELIAL NEOPLASIA; DNA; WOMEN; CANCER; CARCINOGENESIS; INFECTION;

D O I：

10.1007/s00705-014-2186-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results with 1238 cervical specimens from female outpatients showed a concordance rate of 94.3 % between the MPHC and Hybrid Capture II assay. The MPHC assay showed an average HRHPV rate of 29.3 % for high-risk populations in populous cities of China. The established MPHC assay could sensitively and specifically detect 13 types of HRHPV and is suitable for large-scale screening, especially in areas where real-time PCR or fluorescence equipment is unavailable.

引用

页码：3365 / 3370

页数：6

共 25 条

[1] Trials and Projects on Cervical Cancer and Human Papillomavirus Prevention in Sub-Saharan Africa
Adefuye, Peter O.
Broutet, Nathalie J.
de Sanjose, Silvia
Denny, Lynette A.
[J]. VACCINE, 2013, 31 : F53 - F59
[2] Carestiato Fernanda N., 2006, Braz J Infect Dis, V10, P331, DOI 10.1590/S1413-86702006000500006
[3] Chaiwongkot A, 2007, ASIAN PAC J CANCER P, V8, P279
[4] The next-generation Hybrid Capture® High-Risk HPV DNA assay on a fully automated platform
Eder, Pau S.
Lou, Jianrong
Huff, John
Macioszek, Jerzy
[J]. JOURNAL OF CLINICAL VIROLOGY, 2009, 45 : S85 - S92
[5] Global Burden of Human Papillomavirus and Related Diseases
Forman, David
de Martel, Catherine
Lacey, Charles J.
Soerjomataram, Isabelle
Lortet-Tieulent, Joannie
Bruni, Laia
Vignat, Jerome
Ferlay, Jacques
Bray, Freddie
Plummer, Martyn
Franceschi, Silvia
[J]. VACCINE, 2012, 30 : F12 - F23
[6] Evaluation of adjunctive HPV testing by Hybrid Capture II® in women with minor cytological abnormalities for the diagnosis of CIN2/3 and cost comparison with colposcopy -: art. no. 23
Guyot, A
Karim, S
Kyi, MS
Fox, J
[J]. BMC INFECTIOUS DISEASES, 2003, 3 (1)
[7] High-risk human papillomavirus testing for monitoring patients treated for high-grade cervical intraepithelial neoplasia
Jeong, Nan Hee
Lee, Nak Woo
Kim, Hai Joong
Kim, Tak
Lee, Kyu Wan
[J]. JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 2009, 35 (04) : 706 - 711
[8] Human Papillomavirus Genotypes and Cofactors Causing Cervical Intraepithelial Neoplasia and Cervical Cancer in Korean Women
Kim, Jongseung
Kim, Bu Kyung
Lee, Chae Hyeong
Seo, Sang Soo
Park, Sang-Yoon
Roh, Ju-Won
[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2012, 22 (09) : 1570 - 1576
[9] A peer comparison program for the quality assurance of human papillomavirus DNA detection using the digene hybrid capture II/Surepath method shows excellent analytic interlaboratory correlation
Kuebler, Diane L.
Illingworth, Andrea
Blenc, Ann Marie
Wilbur, David C.
[J]. CANCER CYTOPATHOLOGY, 2007, 111 (05): : 339 - 343
[10] Molecular Mechanisms of Human Papillomavirus-Induced Carcinogenesis
Lehoux, Michael
D'Abramo, Claudia M.
Archambault, Jacques
[J]. PUBLIC HEALTH GENOMICS, 2009, 12 (5-6) : 268 - 280

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