A novel fluorescent method for determination of peroxynitrite using folic acid as a probe

被引：69
|
作者：
Huang, Jun-Chao
Li, De-Jia
Diao, Jun-Chen
Hou, Jie
Yuan, Jiang-Lan
Zou, Guo-Lin [1 ]
机构：
[1] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China
[2] Zhengzhou Univ, Coll Med, Henan 450052, Peoples R China
来源：
TALANTA | 2007年 / 72卷 / 04期
基金：
中国国家自然科学基金;
关键词：
peroxynitrite; folic acid; fluorimetry; probe;
D O I：
10.1016/j.talanta.2007.01.033
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel method for the determination of peroxynitrite using folic acid as a fluorescent probe is described. The method is based on the oxidation of the reduced, low-fluorescent folic acid by peroxynitrite to produce a high-fluorescent emission product. The fluorescence increase is linearly related to the concentration of peroxynitrite in the range of 3 x 10(-8) to 5.0 x 10(-6) mol L-1 with a correlation coefficient of 0.998, and the detection limit is 1 x 10(-8) mol L-1. Interferences from some metal ions normally seen in biological samples, and also some anions structurally similar to peroxynitrite were studied. The optimal conditions for the detection of peroxynitrite were evaluated. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：1283 / 1287
页数：5
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