Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture:: identification of a novel wax ester

被引：59
作者：
Bacon, Joanna [1 ]
Dover, Lynn G.
Hatch, Kim A.
Zhang, Yi
Gomes, Jessica M.
Kendal, Sharon
Wernisch, Lorenz
Stoker, Neil G.
Butcher, Philip D.
Besra, Gurdyal S.
Marsh, Philip D.
机构：
[1] Hlth Protect Agcy, TB Res Grp, Ctr Emergency Preparedness & Response, Salisbury SP4 0JG, Wilts, England
[2] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
[3] Univ London, Birkbeck Coll, Sch Crystallog, London WC1E 7HX, England
[4] Univ London Royal Vet Coll, Dept Pathol & Infect Dis, London NW1 0TU, England
[5] St Georgese Hosp, Sch Med, Dept Cellular & Mol Med, Bacterial Microarray Grp, London SW17 0RE, England
来源：
MICROBIOLOGY-SGM | 2007年 / 153卷
基金：
英国医学研究理事会;
关键词：
D O I：
10.1099/mic.0.2006/004317-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menacluinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment.
引用
收藏
页码：1435 / 1444
页数：10
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