A one step derivatization of controlled pore glass for oligonucleotide solid-phase synthesis

被引：8
作者：
Laurent, A
de Lambert, B
Charreyre, MT
Mandrand, B
Chaix, C
机构：
[1] Apibio Zone Astec, F-38054 Grenoble 09, France
[2] Ecole Normale Super Lyon, Unite Mixte BioMerieux, CNRS, F-69364 Lyon 07, France
来源：
TETRAHEDRON LETTERS | 2004年 / 45卷 / 48期
关键词：
diol adsorption; solid-phase synthesis; oligonucleotide; CPG;
D O I：
10.1016/j.tetlet.2004.09.167
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A one step anchoring of various diols on bare controlled pore glass (CPG) support via an adsorption mechanism allowed us to synthesize different (oligonucleotide-3'-diol) conjugates with high purity, through standard phosphoramidite chemistry. Diol loading on CPG proved to be efficient and reproducible. This methodology virtually allows the synthesis of any 3'-modified oligonucleotide, using any reporter molecule containing a diol moiety adsorbed on the CPG. Moreover, vicinal diol as glycol or glycerol enabled the release of some part of oligonucleotide-3'-OH. (C) 2004 Elsevier Ltd. All rights reserved.
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收藏
页码：8883 / 8887
页数：5
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