Characterization of the Binding Interaction between the Oncoprotein Gankyrin and a Grafted 56 ATPase

被引：5

作者：

Chapman, Alex M. ^{[1
]}

Rogers, Bryce E. ^{[1
]}

McNaughton, Brian R. ^{[1
,2
]}

机构：

[1] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Dept Biochem & Mol Biol, Ft Collins, CO 80523 USA

来源：

BIOCHEMISTRY | 2014年 / 53卷 / 44期

基金：

美国国家卫生研究院;

关键词：

BREAST-CANCER; OVEREXPRESSION; PROTEINS; PROMOTES;

D O I：

10.1021/bi5012354

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complex with the C-terminal portion of the proteosomal subunit S6 ATPase is the only available structure of a protein-protein interaction involving the oncoprotein gankyrin. However, difficulties associated with recombinant expression of S6 ATPase alone, or truncations thereof, have limited our understanding of this assembly. We replaced the C-terminal portion of FtsH from Escherichia coli with the structurally homologous C-terminal portion of S6 ATPase and used this grafted protein to characterize the gankyrin-S6 ATPase binding interaction by isothermal titration calorimetry.

引用

页码：6857 / 6859

页数：3

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