Identification of saliva-regulated genes of Streptococcus gordonii DL1 by differential display using random arbitrarily primed PCR

被引:48
作者
Dû, LD [1 ]
Kolenbrander, PE [1 ]
机构
[1] NIDCR, Oral Infect & Immun Branch, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1128/IAI.68.8.4834-4837.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Attachment of Streptococcus gordonii to the acquired pellicle of the tooth surface involves specific interactions between bacterial adhesins and adsorbed salivary components. To study saliva-regulated gene expression in S. gordonii, we used random arbitrarily primed PCR (RAP-PCR). Bacteria were incubated in either brain heart infusion medium or saliva. Total RNA from both conditions was purified and RAP fingerprinted and then PCR amplified with an arbitrary primer. The differentially displayed DNA fragments were cloned, sequenced, and analyzed using the BLAST search network service. Three DNA products were up-regulated. One was identified as that of the sspA and -B genes, which encode the salivary agglutinin glycoprotein-binding proteins SspA and SspB of S. gordonii; another had 79% identity with the Lactococcus lactis clpE gene, encoding a member of the Clp protease family; and the third product showed no significant homology to known genes. Five downregulated genes were identified which encode proteins involved in bacterial metabolism. We have shown, for the first time, direct induction of sspA and -B in S. gordonii by human saliva.
引用
收藏
页码:4834 / 4837
页数:4
相关论文
共 31 条
[1]   Identification of a Streptococcus gordonii SspB domain that mediates adhesion to Porphyromonas gingivalis [J].
Brooks, W ;
Demuth, DR ;
Gil, S ;
Lamont, RJ .
INFECTION AND IMMUNITY, 1997, 65 (09) :3753-3758
[2]  
CHEN NY, 1993, J BIOL CHEM, V268, P9448
[3]   CELL-TO-CELL INTERACTION OF STREPTOCOCCUS-SANGUIS AND PROPIONIBACTERIUM-ACNES ON SALIVA-COATED HYDROXYAPATITE [J].
CIARDI, JE ;
MCCRAY, GFA ;
KOLENBRANDER, PE ;
LAU, A .
INFECTION AND IMMUNITY, 1987, 55 (06) :1441-1446
[4]   SALIVA-MEDIATED AGGREGATION OF ENTEROCOCCUS-FAECALIS TRANSFORMED WITH A STREPTOCOCCUS-SANGUIS GENE ENCODING THE SSP-5 SURFACE-ANTIGEN [J].
DEMUTH, DR ;
BERTHOLD, P ;
LEBOY, PS ;
GOLUB, EE ;
DAVIS, CA ;
MALAMUD, D .
INFECTION AND IMMUNITY, 1989, 57 (05) :1470-1475
[5]   Interruption of the Streptococcus gordonii M5 sspA/sspB intergenic region by an insertion sequence related to IS1167 of Streptococcus pneumoniae [J].
Demuth, DR ;
Duan, Y ;
Jenkinson, HF ;
McNab, R ;
Gil, S ;
Lamont, RJ .
MICROBIOLOGY-UK, 1997, 143 :2047-2055
[6]   Tandem genes encode cell-surface polypeptides SspA and SspB which mediate adhesion of the oral bacterium Streptococcus gordonii to human and bacterial receptors [J].
Demuth, DR ;
Duan, Y ;
Brooks, W ;
Holmes, AR ;
McNab, R ;
Jenkinson, HF .
MOLECULAR MICROBIOLOGY, 1996, 20 (02) :403-413
[7]   CLONING AND EXPRESSION OF A STREPTOCOCCUS-SANGUIS SURFACE-ANTIGEN THAT INTERACTS WITH A HUMAN SALIVARY AGGLUTININ [J].
DEMUTH, DR ;
DAVIS, CA ;
CORNER, AM ;
LAMONT, RJ ;
LEBOY, PS ;
MALAMUD, D .
INFECTION AND IMMUNITY, 1988, 56 (09) :2484-2490
[8]   COMPARISON OF STREPTOCOCCUS-MUTANS AND STREPTOCOCCUS-SANGUIS RECEPTORS FOR HUMAN SALIVARY AGGLUTININ [J].
DEMUTH, DR ;
LAMMEY, MS ;
HUCK, M ;
LALLY, ET ;
MALAMUD, D .
MICROBIAL PATHOGENESIS, 1990, 9 (03) :199-211
[9]   A multilocus sequence typing scheme for Streptococcus pneumoniae:: identification of clones associated with serious invasive disease [J].
Enright, MC ;
Spratt, BG .
MICROBIOLOGY-UK, 1998, 144 :3049-3060
[10]   IDENTIFICATION OF PROTEIN CODING REGIONS BY DATABASE SIMILARITY SEARCH [J].
GISH, W ;
STATES, DJ .
NATURE GENETICS, 1993, 3 (03) :266-272