Inactivation of Cu,Zn-superoxide dismutase by intermediates of Maillard reaction and glycolytic pathway and some sugars

被引：32

作者：

Ukeda, H ^{[1
]}

Hasegawa, Y ^{[1
]}

Ishi, T ^{[1
]}

Sawamura, M ^{[1
]}

机构：

[1] Kochi Univ, Fac Agr, Dept Bioresources Sci, Nankoku 783, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 12期

关键词：

superoxide dismutase; glycation; Maillard reaction; inactivation; glycolytic pathway;

D O I：

10.1271/bbb.61.2039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human Cu,Zn-superoxide dismutase (SOD) was incubated with various intermediates of the Maillard reaction and glycolytic pathway (arabinose, glyoxal, glycolaldehyde, glyceraldehyde, glyceraldehyde 3-phosphate, and dihydroxyacetone) and some reducing sugars (sorbose, xylose, and ribose), The change of the activity and the molecular weight were measured and compared with that of SOD incubated with glucose or fructose, Sorbose, xylose, and ribose decreased the activity with a rate comparable to fructose. Site-specific and random fragmentation were observed upon the incubation with them, Arabinose showed a similar inactivation rate as glucose. The intermediates other than arabinose had a high inactivation rate. Especially, glyceraldehyde, glycolaldehyde, and glyoxal most strongly lowered the activity in a concentration-dependent manner and a significant inactivation was recognized even at 1 mM level, SDS-PAGE band patterns indicated that the inactivation by those carbonyl compounds occurred by both crosslinking and site-specific fragmentation of SOD.

引用

页码：2039 / 2042

页数：4

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