Simultaneous visualization of two Citrus tristeza virus genotypes provides new insights into the structure of multi-component virus populations in a host

被引：11
作者：
Bergua, Maria [1 ,3 ]
Phelan, Dane M. [2 ]
Bak, Aurelie [1 ,3 ]
Bloom, David C. [2 ]
Folimonova, Svetlana Y. [1 ]
机构：
[1] Univ Florida, Dept Plant Pathol, 2550 Hull Rd, Gainesville, FL 32611 USA
[2] Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL 32603 USA
[3] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
来源：
VIROLOGY | 2016年 / 491卷
基金：
美国国家科学基金会;
关键词：
Virus populations; Citrus tristeza virus; RNA in situ hybridization; PLUM-POX-VIRUS; MOSAIC-VIRUS; SUPERINFECTION EXCLUSION; MOLECULAR VARIABILITY; MONOCLONAL-ANTIBODIES; GENETIC DIVERSITY; PLANT-VIRUSES; RNA; EVOLUTION; RECOMBINATION;
D O I：
10.1016/j.virol.2016.01.017
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Complex Citrus tristeza virus (CTV) populations composed of mixtures of different strains of the virus are commonly found in citrus trees in the field. At present, little is known about how these populations are formed, maintained, and how they are structured within a host. Here we used a novel in situ hybridization approach allowing simultaneous visualization of two different RNA targets with high sensitivity and specificity to examine the distribution of two isolates, T36 and T68-1, representing phylogenetically distinct strains of CTV, in a citrus host in single and mixed infections. Remarkably, in doubly inoculated plants the two virus variants appeared to be well mixed within the infected tissue and showed no spatial segregation. In addition, both CTV variants were often found occupying the same cells. Possible mechanisms involved in shaping CTV populations and the biological significance of the observed lack of structural separation of the individual components are discussed. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：10 / 19
页数：10
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