Enhanced cellulase production of the Trichoderma viride mutated by microwave and ultraviolet

被引：74

作者：

Li, Xing-hua ^{[1
]}

Yang, Hua-jun ^{[1
]}

Roy, Bhaskar ^{[1
]}

Park, Enoch Y. ^{[2
]}

Jiang, Li-jun ^{[1
]}

Wang, Dan ^{[1
]}

Miao, Yun-gen ^{[1
]}

机构：

[1] Zhejiang Univ, Key Lab Anim Epidem Etiol & Immunol Prevent, Minist Agr, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China

[2] Shizuoka Univ, Biotechnol Lab, Dept Appl Biol Chem, Fac Agr, Shizuoka 4228529, Japan

来源：

MICROBIOLOGICAL RESEARCH | 2010年 / 165卷 / 03期

关键词：

Cellulase; Trichoderma viride; Mutation; Microwave; Ultraviolet; REESEI; ETHANOL; FERMENTATION; PURIFICATION; CULTIVATION; BIOMASS; GENES; XYLAN;

D O I：

10.1016/j.micres.2009.04.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cellulase-producing fungi Trichoderma viride were cultured and fermented on the solid-state wheat bran fermentation medium. The characteristics of its carboxymethyl cellulase (CMCase) in the condition of this solid-state fermentation were evaluated, and the optimum culture time, optimum pH and optimum temperature for CMCase activity of T viride fermented in this solid state were 60 h, 5.0 and 50 degrees C, respectively. Carboxymethyl cellulose sodium (CMC-Na) and Congo red were used to screen the strains that had stronger ability to produce enzymes. After the compound mutagenesis by microwave and ultraviolet, seven mutant strains (M-B1-M-B7) were selected and their CMCase activities were assayed. Five of them (M-B1, M-B2, M-B3, M-B5 and M-B7) had significantly stronger ability to produce enzymes than the normal wild type, and they were also very stable for a long period up to 9 generations to produce cellulase. Molecular studies showed that there were some base mutations in endoglucanase I (EG I) genes of mutants M-B1, M-B2, M-B3 and M-B5, but no change in M-B7, suggesting that some amino mutations in EG I proteins caused by base mutations could lead to enhanced cellulase production. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：190 / 198

页数：9

共 18 条

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