Nm-seq maps 2′-O-methytation sites in human mRNA with base precision

被引：219

作者：

Dai, Qing ^{[1
,2
]}

Moshitch-Moshkovitz, Sharon ^{[3
,4
]}

Han, Dali ^{[1
,2
]}

Kol, Nitzan ^{[3
]}

Amariglio, Ninette ^{[3
,4
,5
]}

Rechavi, Gideon ^{[3
,4
,6
]}

Dominissini, Dan ^{[3
,4
,6
]}

He, Chuan ^{[1
,2
,7
,8
]}

机构：

[1] Univ Chicago, Dept Chem, 5735 S Ellis Ave, Chicago, IL 60637 USA

[2] Univ Chicago, Howard Hughes Med Inst, 5841 S Maryland Ave, Chicago, IL 60637 USA

[3] Chaim Sheba Med Ctr, Canc Res Ctr, Tel Hashomer, Israel

[4] Chaim Sheba Med Ctr, Wohl Ctr Translat Med, Tel Hashomer, Israel

[5] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, Ramat Gan, Israel

[6] Tel Aviv Univ, Sackler Sch Med, Tel Aviv, Israel

[7] Univ Chicago, Dept Biochem & Mol Biol, 920 E 58th St, Chicago, IL 60637 USA

[8] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

来源：

NATURE METHODS | 2017年 / 14卷 / 07期

基金：

美国国家卫生研究院;

关键词：

RIBOSOMAL-RNA; METHYLATION; CAP; THERMODYNAMICS; TRANSLATION; ELIMINATION; BIOGENESIS; COMPLEXES; PROTEINS; SEQUENCE;

D O I：

10.1038/nmeth.4294

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

引用

页码：695 / +

页数：6

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