Principles and challenges of genome-wide DNA methylation analysis

被引：1077

作者：

Laird, Peter W. ^{[1
]}

机构：

[1] Univ So Calif, Keck Sch Med, USC Epigenome Ctr, Los Angeles, CA 90089 USA

来源：

NATURE REVIEWS GENETICS | 2010年 / 11卷 / 03期

关键词：

CPG-ISLAND METHYLATION; CYTOSINE-METHYLATION; BREAST-CANCER; COLORECTAL-CANCER; UTILITY PROGRAM; IMPRINTED GENE; WHOLE-GENOME; T-CELLS; IDENTIFICATION; REVEALS;

D O I：

10.1038/nrg2732

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Methylation of cytosine bases in DNA provides a layer of epigenetic control in many eukaryotes that has important implications for normal biology and disease. Therefore, profiling DNA methylation across the genome is vital to understanding the influence of epigenetics. There has been a revolution in DNA methylation analysis technology over the past decade: analyses that previously were restricted to specific loci can now be performed on a genome-scale and entire methylomes can be characterized at single-base-pair resolution. However, there is such a diversity of DNA methylation profiling techniques that it can be challenging to select one. This Review discusses the different approaches and their relative merits and introduces considerations for data analysis.

引用

页码：191 / 203

页数：13

共 174 条

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