Development of a real-time PCR assay for the direct detection of Candida species causing Vulvovaginal candidiasis

被引：4
作者：
Tardif, Keith D. [1 ]
Schlaberg, Robert [1 ,2 ]
机构：
[1] ARUP Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
[2] Univ Utah, Sch Med, Dept Pathol, Salt Lake City, UT USA
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 88卷 / 01期
关键词：
Candidiasis; Vulvovaginal; PCR; Assay development; Diagnostic; ANTIFUNGAL SUSCEPTIBILITY; FLUCONAZOLE PROPHYLAXIS;
D O I：
10.1016/j.diagmicrobio.2017.01.012
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Identification of Candida species by traditional methods can be time-consuming and have limited analytical sensitivity. We developed a multiplex real-time PCR assay for detection and differentiation of Candida species causing vulvovaginal candidiasis (VVC). Overall, this PCR assay is a powerful diagnostic tool offering superior accuracy, sensitivity, and specificity. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：39 / 40
页数：2
相关论文
共 50 条
  • [31] Detection of monkeypox virus with real-time PCR assays
    Li, Yu
    Olson, Victoria A.
    Laue, Thomas
    Laker, Miriam T.
    Damon, Inger K.
    JOURNAL OF CLINICAL VIROLOGY, 2006, 36 (03) : 194 - 203
  • [32] Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR
    Haque, F.
    Fisseha, S.
    Athamanolap, P.
    Tower, R.
    Ortega, J.
    Dominguez, C.
    Maruca, T.
    Torpey, D.
    Myers, R.
    Laksanalamai, P.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2020, 178
  • [33] Development of two real-time PCR assays for the detection of Mycoplasma hyopneumoniae in clinical samples
    Dubosson, CR
    Conzelmann, C
    Miserez, R
    Boerlin, P
    Frey, J
    Zimmermann, W
    Häni, H
    Kuhnert, P
    VETERINARY MICROBIOLOGY, 2004, 102 (1-2) : 55 - 65
  • [34] COMPARATIVE EVALUATION OF REAL-TIME PCR WITH CONVENTIONAL PCR ASSAY FOR DIAGNOSIS OF BRUCELLOSIS IN RUMINANTS
    Saleem, M. Z.
    Akhtar, R.
    Aslam, A.
    Rashid, M., I
    Chaudhry, Z., I
    Shah, B. A.
    Ahmed, R.
    Akmal, M.
    Murtaza, G.
    JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS, 2020, 30 (06): : 1389 - 1394
  • [35] PCR and real-time PCR assays to detect fungi of Alternaria alternata species
    Kordalewska, Milena
    Brillowska-Dabrowska, Anna
    Jagielski, Tomasz
    Dworecka-Kaszak, Bozena
    ACTA BIOCHIMICA POLONICA, 2015, 62 (04) : 707 - 712
  • [36] Development of Direct Real-Time PCR System Applicable to a Wide Range of Foods and Agricultural Products
    Mano, Junichi
    Hatano, Shuko
    Futo, Satoshi
    Minegishi, Yasutaka
    Ninomiya, Kenji
    Nakamura, Kosuke
    Kondo, Kazunari
    Teshima, Reiko
    Takabatake, Reona
    Kitta, Kazumi
    FOOD HYGIENE AND SAFETY SCIENCE, 2014, 55 (01): : 25 - 33
  • [37] A nested real-time PCR assay has an increased sensitivity suitable for detection of viruses in aerosol studies
    Perrott, P.
    Smith, G.
    Ristovski, Z.
    Harding, R.
    Hargreaves, M.
    JOURNAL OF APPLIED MICROBIOLOGY, 2009, 106 (05) : 1438 - 1447
  • [38] DETECTION OF VIRUSES INFECTING BLACK PEPPER BY SYBR GREEN-BASED REAL-TIME PCR ASSAY
    Bhat, A. I.
    Siljo, A.
    JOURNAL OF PLANT PATHOLOGY, 2014, 96 (01) : 105 - 109
  • [39] A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses
    Shi, Xiju
    Liu, Xuming
    Wang, Qin
    Das, Amaresh
    Ma, Guiping
    Xu, Lu
    Sun, Qing
    Peddireddi, Lalitha
    Jia, Wei
    Liu, Yanhua
    Anderson, Gary
    Bai, Jianfa
    Shi, Jishu
    JOURNAL OF VIROLOGICAL METHODS, 2016, 236 : 258 - 265
  • [40] Dual-probe real-time PCR assay for detection of variola or other orthopoxviruses with dried reagents
    Aitichou, Mohamed
    Saleh, Sharron
    Kyusung, Park
    Huggins, John
    O'Guinn, Monica
    Jahrling, Peter
    Ibrahim, Sofi
    JOURNAL OF VIROLOGICAL METHODS, 2008, 153 (02) : 190 - 195
12345