Detecting acetyl-coenzyme a carboxylase resistance gene in rice (Oryza sativa L.)

被引:5
|
作者
Pereira, Adriana [1 ]
Tcacenco, Fernando Adami [2 ]
Klabunde, Gustavo Henrique Ferrero [1 ]
de Andrade, Alexander [1 ]
机构
[1] Empresa Pesquisa Agr & Extensao Rural Santa Catar, EEI, Rodovia Antonio Heil 6800, Itajai, SC, Brazil
[2] Univ Vale Itajai Univali, Rua Uruguai 458, Itajai, SC, Brazil
关键词
ACCase inhibitors; Allele-specific PCR; Herbicide resistance; Primer design; Rice breeding; PCR ASSAYS; MUTATIONS; IMPACT;
D O I
10.1007/s11033-019-05068-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Herbicides inhibiting acetyl-coenzyme A carboxylase (ACCase) are very effective in controlling grass weeds including weedy-rice in paddy rice production systems. The ACCase inhibitor affects the enzyme by blocking fatty acid biosynthesis resulting in plant death. The herbicide resistance in rice is conferred by a single point mutation with an amino acid substitution of the carboxyl transferase domain of the ACCase gene. An assay based on the tetra-primer ARMS-PCR method was developed to detect the SNP G2027T that causes a tryptophan-cysteine substitution in the gene encoding chloroplastic ACCase in rice. The protocol was tested in 453 rice samples from a segregant population for validation of the assay. This technique can be exploited to monitor resistant lines in rice breeding programs to detect homozygous or heterozygous resistant genotypes and homozygous susceptible genotypes. The presence of resistant ACCase allele(s) can be detected with rapidity, simplicity, at low cost and can be used in any molecular biology laboratory with minimal equipment.
引用
收藏
页码:6271 / 6276
页数:6
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