Inorganic nanoparticles as potential regulators of immune response in dendritic cells

被引：37

作者：

Fogli, Silvia ^{[1
,2
]}

Montis, Costanza ^{[1
,2
]}

Paccosi, Sara ^{[3
]}

Silvano, Angela ^{[4
]}

Michelucci, Elena ^{[5
]}

Berti, Debora ^{[1
,2
]}

Bosi, Alberto ^{[4
]}

Parenti, Astrid ^{[3
]}

Romagnoli, Paolo ^{[4
]}

机构：

[1] Univ Florence, Dept Chem Ugo Schiff, Via Lastruccia 3, I-50019 Sesto Fiorentino, FI, Italy

[2] CSGI, Via Lastruccia 3, I-50019 Sesto Fiorentino, FI, Italy

[3] Univ Florence, Dept Hlth Sci, Clin Pharmacol & Oncol Sect, Viale Pieraccini 6, I-50139 Florence, FI, Italy

[4] Univ Florence, Dept Expt & Clin Med, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, FI, Italy

[5] Univ Florence, Mass Spectrometry Ctr CISM, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, IF, Italy

来源：

NANOMEDICINE | 2017年 / 12卷 / 14期

关键词：

cancer immunotherapy; dendritic cells; lysate; nanoparticles; protein corona; PROTEIN CORONA; CANCER VACCINES; GOLD NANOPARTICLES; DRUG-DELIVERY; SIZE; SURFACE; IMMUNOTHERAPY; ENDOCYTOSIS; ADSORPTION; IMPACT;

D O I：

10.2217/nnm-2017-0061

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: The spontaneous adsorption of proteins on nanoparticles (NPs) in biological media is exploited to prepare complexes of NPs and proteins from cancer cells' lysates for application in cancer immunotherapy. Materials & methods: Gold (Au) and silica NPs were synthesized, incubated with cancer cells' lysates and characterized. Dendritic cells (DCs) were challenged with protein-coated NPs, their maturation, viability and morphology were evaluated and lymphocytes T proliferation was determined. Results: Silica and Au NPs bound different pools of biomolecules from lysates, and are therefore promising selective carriers for antigens. When incubated with immature DCs, NPs were efficiently endocytosed without cytotoxicity. Finally, protein-coated AuNPs promoted DC maturation and DC-mediated lymphocyte proliferation, at variance with lysate alone and protein-coated silica NPs, that did not promote DCs maturation. Conclusion: These results demonstrate that the spontaneous formation of protein coronas on NPs represents a possible approach to fast, easy, cost-effective DCs stimulation.

引用

页码：1647 / 1660

页数：14