Spectroscopic and Permeation Studies of Phospholipid Bilayers Supported by a Soft Hydrogel Scaffold

被引：5
作者：
Grossutti, Michael [1 ]
Seenath, Ryan [1 ]
Conlon, Shannon [1 ]
Leitch, J. Jay [1 ]
Li, Jie [1 ]
Lipkowski, Jacek [1 ]
机构：
[1] Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada
来源：
LANGMUIR | 2014年 / 30卷 / 36期
基金：
加拿大创新基金会; 加拿大自然科学与工程研究理事会;
关键词：
AU(111) ELECTRODE SURFACE; REFLECTION-ABSORPTION-SPECTROSCOPY; LIPID-COATED MICROGELS; PM-IRRAS; INFRARED-SPECTROSCOPY; DRUG-DELIVERY; MEMBRANES; CHOLESTEROL; ORIENTATION; LIPOBEADS;
D O I：
10.1021/la502925p
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Polarized attenuated total reflection infrared (ATR-IR) spectroscopy, fluorescence microscopy, and fluorescence spectroscopy were used to characterize a lipid coating composed of 70 mol % 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 30 mol % cholesterol, supported on a spherical hydrogel scaffold. The fluorescence microscopy images show an association between the lipid coating and the hydrogel scaffold. Fluorescence permeability measurements revealed that the phospholipid coating acts as a permeability barrier, exhibiting characteristics of a lamellar bilayer coating structure. Variable evanescent wave penetration depth ATR-IR spectroscopy studies validated the determination of quantitative molecular orientation information for a lipid coating supported on a spherical scaffold. These polarized ATR-IR studies measured an average DMPC acyl chain tilt angle of similar to 21-25 degrees, with respect to the surface normal.
引用
收藏
页码：10862 / 10870
页数：9
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