Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release

被引:18
作者
Victoni, Tatiana [1 ,2 ]
Salvator, Helene [2 ,3 ]
Abrial, Charlotte [2 ]
Brollo, Marion [2 ]
Sobrino Porto, Luis Cristovao [1 ]
Lagente, Vincent [6 ]
Naline, Emmanuel [2 ,3 ]
Grassin-Delyle, Stanislas [3 ,4 ,5 ]
Devillier, Philippe [2 ,3 ]
机构
[1] Lab Tissue Repair, Lab Histocompatibil & Cryopresevat, Rio De Janeiro, Brazil
[2] Univ Paris Saclay, Univ Versailles St Quentin, UFR Sci Sante Simone Veil, Lab Res Resp Pharmacol UPRES EA220, 11 Rue Guillaume Lenoir, F-92150 Suresnes, France
[3] Foch Hosp, Dept Airway Dis, Suresnes, France
[4] Univ Paris Saclay, Univ Versailles St Quentin, UFR Sci Sante Simone Veil, INSERM UMR1173, Montigny Le Bretonneux, France
[5] Univ Paris Saclay, Univ Versailles St Quentin, UFR Sci Sante Simone Veil, Mass Spectrometry Facil, Montigny Le Bretonneux, France
[6] Univ Bretagne Loire, Univ Rennes 1, INRA, INSERM,Nutr Metab & Canc, Rennes, France
来源
RESPIRATORY RESEARCH | 2017年 / 18卷
关键词
beta(2)-adrenoceptor; Cytokines; Lipopolysaccharide; Lung macrophage; Monocyte-derived macrophage; HUMAN ALVEOLAR MACROPHAGES; OBSTRUCTIVE PULMONARY-DISEASE; BLOOD MONONUCLEAR-CELLS; NECROSIS-FACTOR-ALPHA; ADRENOCEPTOR AGONISTS; BETA-ADRENOCEPTORS; INNATE IMMUNITY; EP4; RECEPTOR; TNF-ALPHA; IN-VITRO;
D O I
10.1186/s12931-017-0613-y
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: beta(2)-adrenoceptor agonists have been shown to reduce the lipopolysaccharide (LPS)-induced cytokine release by human monocyte-derived macrophages (MDMs). We compare the expression of beta(2)-adrenoceptors and the inhibitory effect of formoterol and salmeterol on the LPS-induced release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6 and a range of chemokines (CCL2, 3, 4, and IL-8) by human lung macrophages (LMs) and MDMs. Methods: LMs were isolated from patients undergoing resection and MDMs were obtained from blood monocytes in the presence of GM-CSF. LMs and MDMs were incubated in the absence or presence of formoterol or salmeterol prior to stimulation with LPS. The effects of formoterol were also assessed in the presence of the phosphodiesterase inhibitor roflumilast. Results: LPS-induced cytokine production was higher in LMs than in MDMs. Salmeterol and formoterol exerted an inhibitory effect on the LPS-induced production of TNF-alpha, IL-6, CCL2, CCL3, and CCL4 in MDMs. In contrast, the beta(2)-adrenoceptor agonists were devoid of any effect on LMs-even in the presence of roflumilast. The expression of beta(2)-adrenergic receptors was detected on Western blots in MDMs but not in LMs. Conclusions: Concentrations of beta(2)-adrenoceptor agonists that cause relaxation of the human bronchus can inhibit cytokine production by LPS-stimulated MDMs but not by LMs.
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页数:10
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