Quantification of V(D)J recombination by real-time quantitative PCR

被引：5

作者：

Braikia, Fatima-Zohra ^{[1
,2
,3
,4
,5
]}

Chemin, Guillaume ^{[1
,2
,3
,4
,5
]}

Moutahir, Mohammed ^{[1
,2
,3
,4
,5
]}

Khamlichi, Ahmed Amine ^{[1
,2
,3
,4
,5
]}

机构：

[1] CNRS UMR 5089, F-31077 Toulouse, France

[2] Inst Pharmacol & Biol Struct, F-31077 Toulouse, France

[3] Univ Toulouse, F-31077 Toulouse, France

[4] UPS, F-31077 Toulouse, France

[5] IPBS, F-31077 Toulouse, France

来源：

IMMUNOLOGY LETTERS | 2014年 / 162卷 / 01期

关键词：

B lymphocyte; IgH locus; V(D)J recombination; Real-time quantitative PCR; SEQUENCE; REGION;

D O I：

10.1016/j.imlet.2014.08.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

B and T lymphocytes have the unique capacity to somatically rearrange their antigen receptor loci through V(D)J recombination. D-J(H) and V-H-DJ(H) recombination events are usually visualized by semi-quantitative PCR followed by detection of end products, which is time consuming and requires the use of hazardous elements. Additionally, it necessitates relatively large amounts of genomic DNA which could be limiting when the cell populations of interest are rare. Here, we describe a real-time quantitative PCR assay for a fast quantification of V(D)J recombination events at the IgH locus. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：119 / 123

页数：5

共 5 条

[1] Complete sequence assembly and characterization of the C57BL/6 mouse Ig heavy chain V region [J].