Determination of protease inhibitors using liquid chromatography-tandem mass spectrometry

被引：49
作者：
Frerichs, VA
DiFrancesco, R
Morse, GD
机构：
[1] Pharmacotherapy Research Center, Department of Pharmacy Practice, University at Buffalo, Buffalo
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 787卷 / 02期
关键词：
protease inhibitors;
D O I：
10.1016/S1570-0232(02)01002-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the analysis of six protease inhibitors and one metabolite has been developed and validated. Amprenavir, ritonavir, saquinavir, lopinavir, indinavir, nelfinavir, and an active metabolite of nelfinavir (M8) are quantitated using reversed-phase liquid chromatography coupled to tandem mass spectrometry, equipped with an electrospray ionization source (ESI-LC-MS-MS). The validation data presented here shows that the method allows the rugged analysis of these species from one aliquot. The evolution of complex drug interactions assessments and the clinical use of therapeutic drug monitoring for these antiretrovirals will be a potential immediate application of this method. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：393 / 403
页数：11
相关论文
共 20 条
[1]  
*AM CHEM SOC, 2001, SCFIND SCH ONL 2001
[2]   Quantitation of SR 27417 in human plasma using electrospray liquid chromatography tandem mass spectrometry: A study of ion suppression [J].
Buhrman, DL ;
Price, PI ;
Rudewicz, PJ .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1996, 7 (11) :1099-1105
[3]   Rapid high-performance liquid chromatography determination of lopinavir, a novel HIV-1 protease inhibitor, in human plasma [J].
Faux, J ;
Venisse, N ;
Olivier, JC ;
Bouquet, S .
CHROMATOGRAPHIA, 2001, 54 (7-8) :469-473
[4]  
FU L, 1998, J PHARM BIOMED ANAL, V18, P347
[5]   Liquid chromatographic-tandem mass spectrometric determination of amprenavir (agenerase) in serum/plasma of human immunodeficiency virus type-1 infected patients receiving combination antiretroviral therapy [J].
Gunawan, S ;
Griswold, MP ;
Kahn, DG .
JOURNAL OF CHROMATOGRAPHY A, 2001, 914 (1-2) :1-4
[6]   Sample preparation for LC/MS/MS: Analyzing biological and environmental samples [J].
Henion, J ;
Brewer, E ;
Rule, G .
ANALYTICAL CHEMISTRY, 1998, 70 (19) :650A-656A
[7]   An LC-MS-MS method for the determination of indinavir, an HIV-1 protease inhibitor, in human plasma [J].
Jayewardene, AL ;
Kearney, B ;
Stone, JA ;
Gambertoglio, JG ;
Aweeka, FT .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2001, 25 (02) :309-317
[8]   FROM IONS IN SOLUTION TO IONS IN THE GAS-PHASE - THE MECHANISM OF ELECTROSPRAY MASS-SPECTROMETRY [J].
KEBARLE, P ;
TANG, L .
ANALYTICAL CHEMISTRY, 1993, 65 (22) :A972-A986
[9]  
KEIL K, 2003, IN PRESS THER DRUG M
[10]   Determination of nelfinavir, a potent HIV protease inhibitor, and its active metabolite M8 in human plasma by high-performance liquid chromatography with photodiode-array detection [J].
Lamotte, C ;
Peytavin, G ;
Farinotti, R .
JOURNAL OF CHROMATOGRAPHY B, 1999, 735 (02) :159-170
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