Titanium With Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway

被引：46
作者：
Castro-Raucci, Larissa M. S. [1 ]
Francischini, Marcelo S. [1 ]
Teixeira, Lucas N. [1 ]
Ferraz, Emanuela P. [1 ]
Lopes, Helena B. [1 ]
de Oliveira, Paulo T. [1 ]
Hassan, Mohammad Q. [2 ]
Rosa, Adalberto L. [1 ]
Beloti, Marcio M. [1 ]
机构：
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP, Brazil
[2] Univ Alabama Birmingham, Inst Oral Hlth Res, Birmingham, AL USA
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2016年 / 117卷 / 07期
基金：
巴西圣保罗研究基金会;
关键词：
BMP; BONE; NANOTOPOGRAPHY; OSTEOBLAST; TITANIUM; STEM-CELL DIFFERENTIATION; TRANSCRIPTIONAL PROGRAM; SURFACE-ROUGHNESS; IN-VITRO; TRANSDUCTION; GENE; PROLIFERATION; REGENERATION; MATURATION; RECEPTORS;
D O I：
10.1002/jcb.25469
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We aimed at evaluating the effect of titanium (Ti) with nanotopography (Nano) on the endogenous expression of BMP-2 and BMP-4 and the relevance of this process to the nanotopography-induced osteoblast differentiation. MC3T3-E1 cells were grown on Nano and machined (Machined) Ti surfaces and the endogenous BMP-2/4 expression and the effect of BMP receptor BMPR1A silencing in both osteoblast differentiation and expression of genes related to TGF-beta/BMP signaling were evaluated. Nano supported higher BMP-2 gene and protein expression and upregulated the osteoblast differentiation compared with Machined Ti surface. The BMPR1A silencing inhibited the osteogenic potential induced by Nano Ti surface as indicated by reduced alkaline phosphatase (ALP), osteocalcin and RUNX2 gene expression, RUNX2 protein expression and ALP activity. In addition, the expression of genes related to TGF-/BMP signaling was deeply affected by BMPR1A-silenced cells grown on Nano Ti surface. In conclusion, we have demonstrated for the first time that nanotopography induces osteoblast differentiation, at least in part, by upregulating the endogenous production of BMP-2 and modulating BMP signaling pathway. (C) 2015 Wiley Periodicals, Inc.
引用
收藏
页码：1718 / 1726
页数：9
相关论文
共 42 条
[1]  
Balloni S, 2009, INT J ORAL MAX IMPL, V24, P627
[2]   Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis [J].
Bandyopadhyay, Amitabha ;
Tsuji, Kunikazu ;
Cox, Karen ;
Harfe, Brian D. ;
Rosen, Vicki ;
Tabin, Clifford J. .
PLOS GENETICS, 2006, 2 (12) :2116-2130
[3]   Activation of the Janus kinase/STAT (signal transducer and activator of transcription) signal transduction pathway by interleukin-6-type cytokines promotes osteoblast differentiation [J].
Bellido, T ;
Borba, VZC ;
Roberson, P ;
Manolagas, SC .
ENDOCRINOLOGY, 1997, 138 (09) :3666-3676
[4]   Use of recombinant human bone morphogenetic protein-2 to achieve posterolateral lumbar spine fusion in humans - A prospective, randomized clinical pilot trial - 2002 Volvo Award in clinical studies [J].
Boden, SD ;
Kang, J ;
Sandhu, H ;
Heller, JG .
SPINE, 2002, 27 (23) :2662-2673
[5]   Bmp signaling regulates a dose-dependent transcriptional program to control facial skeletal development [J].
Bonilla-Claudio, Margarita ;
Wang, Jun ;
Bai, Yan ;
Klysik, Elzbieta ;
Selever, Jennifer ;
Martin, James F. .
DEVELOPMENT, 2012, 139 (04) :709-719
[6]   Role of material surfaces in regulating bone and cartilage cell response [J].
Boyan, BD ;
Hummert, TW ;
Dean, DD ;
Schwartz, Z .
BIOMATERIALS, 1996, 17 (02) :137-146
[7]   Osteoblasts Display Different Responsiveness to TRAIL-Induced Apoptosis During Their Differentiation Process [J].
Brunetti, Giacomina ;
Oranger, Angela ;
Carbone, Claudia ;
Mori, Giorgio ;
Sardone, Francesca Rita ;
Mori, Claudio ;
Celi, Monica ;
Faienza, Maria Felicia ;
Tarantino, Umberto ;
Zallone, Alberta ;
Grano, Maria ;
Colucci, Silvia .
CELL BIOCHEMISTRY AND BIOPHYSICS, 2013, 67 (03) :1127-1136
[8]  
Bueno Renan de Barros e Lima, 2011, Braz. Dent. J., V22, P179
[9]   Bone morphogenetic proteins [J].
Chen, D ;
Zhao, M ;
Mundy, GR .
GROWTH FACTORS, 2004, 22 (04) :233-241
[10]   Adenovirus encoding BMP-7 immobilized on titanium surface exhibits local delivery ability and regulates osteoblast differentiation in vitro [J].
Chen, Shenyuan ;
Yang, Jiahua ;
Wang, Hang ;
Chao, Yonglie ;
Zhang, Chaoliang ;
Shen, Jiefei ;
Zhang, Ping .
ARCHIVES OF ORAL BIOLOGY, 2013, 58 (09) :1225-1231
12345