High-pressure liquid chromatography in lab-on-a-chip devices

被引：26

作者：

Ehlert, Steffen ^{[1
]}

Tallarek, Ulrich ^{[1
]}

机构：

[1] Univ Magdeburg, Inst Verfahrenstech, D-39106 Magdeburg, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 388卷 / 03期

关键词：

FLOW; GENERATION; DISPERSION; MICROCHIP; INJECTOR; SYSTEMS;

D O I：

10.1007/s00216-007-1149-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A study was conducted to investigate the use of high-pressure liquid chromatography (LC) in lab-on-a-chip devices. The use of high-pressure liquid chromatography was effective in generating significant analyses, enabling a variety of of chip morphologies to be designed to conduct chemical reaction, mixing, and multidimensional separations. The use of high-pressure liquid chromatography in lab--on-a-chip devices included, the integration of individual operations, such as reaction, preconcentration, and separation in mass-produced, low-cost devices, and automation. The microchip was also hydraulically interfaced with a nano-LC high-pressure pump and an autosampler through a face-seal rotary valve, to perform regular analysis up to 150 bar. The rotary valve provided a means for switching between sample loading and separation configurations, with minimum dead and delay volumes, while allowing high-pressure operation.

引用

页码：517 / 520

页数：4

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