The probiotic effects of Lactobacillus acidophilus and Lactobacillus rhamnosus (probiotic blend) in fish have been documented recently. In this study, the effects of different levels of the probiotic blend on the growth performance, histopathological changes of Labeo rohita fingerlings were evaluated. Fish was fed with basal diet containing formulated feed (control), Exp-I-0.5gm, Exp-II-1gm, Exp-III-1.5gm probiotic blend for 60 days. Growth performance indices and histopathological changes were examined at 60 days of study. Growth performance is evaluated on days 30 and 60 of studying. Fish fingerling samples were taken at the same days of study and histological study including Gill, Liver, Muscle, and kidney effects are compared among the control. Results showed that oral administration of probiotic blend for 60 days had a significant impact on the growth rate of Labeo rohita fingerlings. Dietary administration of probiotic blend (Exp-III) increased considerably the growth rate compared to the control at 30 days of study. However, Exp-III showed significantly increased in growth rate compared to the control at day 60. Similarly, histopathological changes (Gill, Liver, Muscle and Kidney) were significantly no effects in Exp-I, Exp-II, Exp-III at day 60 of the study compared to the control. These results suggest that dietary supplementation of food with 1.5gm probiotic concentration in day 60 is suitable for enhancing the growth and histopathological changes of (Labeo rohita) rohu fish fingerlings.