Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin

被引：10

作者：

Ismail, Fatma ^{[1
]}

Adeloju, Samuel B. ^{[1
]}

机构：

[1] Monash Univ, NanoSci & Sensor Technol Res Grp, Sch Appl Sci & Engn, Churchill, Vic 3842, Australia

来源：

SENSORS | 2010年 / 10卷 / 04期

关键词：

penicillin; penicillinase; polytyramine; biosensor; potentiometry; GLUCOSE-OXIDASE; ELECTROCHEMICAL POLYMERIZATION; ELECTRODEPOSITED POLYTYRAMINE; ELECTRON-TRANSFER; BIOSENSOR ASSAY; ENZYME; IMMOBILIZATION; CARBON; FERROCENE; MILK;

D O I：

10.3390/s100402851

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3, and 3 mM penicillin with an applied current density of 0.8 mA/cm(2) and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3-283 mu M for penicillin and achieved a minimum detectable concentration of 0.3 mu M. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 +/- 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are >= 20 ppm.

引用

页码：2851 / 2868

页数：18

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