Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence

被引：55

作者：

Li, Chunqiang ^{[1
,2
]}

Pastila, Riikka K. ^{[1
,2
,3
]}

Pitsillides, Costas ^{[1
,2
,4
]}

Runnels, Judith M. ^{[1
,2
,5
]}

Puoris'haag, Mehron ^{[1
,2
]}

Cote, Daniel ^{[6
]}

Lin, Charles P. ^{[1
,2
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed, Boston, MA 02115 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Syst Biol, Boston, MA USA

[3] Radiat & Nucl Safety Author, FI-00881 Helsinki, Finland

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA

[6] Univ Laval, Univ Laval Robert Giffard, Ctr Rech, Quebec City, PQ G1J2G3, Canada

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 02期

基金：

美国国家卫生研究院; 芬兰科学院;

关键词：

LASER-SCANNING MICROSCOPY; NONMELANOMA SKIN-CANCER; ULTRAVIOLET MICROSCOPY; MULTIPHOTON MICROSCOPY; CONFOCAL MICROSCOPY; 3-PHOTON EXCITATION; BONE-MARROW; INFLAMMATION; MIGRATION; PROTEINS;

D O I：

10.1364/OE.18.000988

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans. (C) 2010 Optical Society of America

引用

页码：988 / 999

页数：12

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