Supercritical CO2 Extraction of Carotenoids (Astaxanthin, Adonirubin, and Adonixanthin) from Paracoccus carotinifaciens: Improved Z-Isomer Ratio and Recovery of Carotenoids via High-Temperature Extraction

被引:4
作者
Honda, Masaki [1 ]
Murakami, Kazuya [1 ]
Osawa, Yukiko [2 ]
Kawashima, Yuki [2 ]
Wasai, Masafumi [2 ]
Hirasawa, Kazuaki [2 ]
Kuroda, Ikuo [2 ]
机构
[1] Meijo Univ, Fac Sci & Technol, Nagoya, Aichi 4688502, Japan
[2] ENEOS Corp, Biotechnol R&D Grp, Yokohama, Kanagawa 2310815, Japan
关键词
astaxanthin; extraction; Paracoccus carotinifaciens; supercritical CO2; thermal isomerization; HAEMATOCOCCUS-PLUVIALIS; GEOMETRICAL-ISOMERS; BETA-CAROTENE; LYCOPENE; OIL; ISOMERIZATION; TRANSPORT; ETHANOL; FLUID;
D O I
10.1002/ejlt.202200021
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The effects of high-temperature supercritical CO2 (SC-CO2) extraction on Z-isomerization and recovery of carotenoids (astaxanthin, adonirubin, and adonixanthin) from Paracoccus carotinifaciens are investigated. The high-temperature extraction, especially at >100 degrees C, not only increases the total Z-isomer ratio of carotenoids in the resulting extracts but also improves carotenoid recovery. For example, when the SC-CO2 extraction is performed at 60 degrees C and 30 MPa with an entrainer (ethanol), the total Z-isomer ratio and the recovery of astaxanthin are 11.1% and 28.4%, respectively, whereas at 120 degrees C, the values increase to 54.2 and 56.4%, respectively. The significant improvement in the extraction efficiency of carotenoids at high temperatures can be attributed to the increase in the ratio of carotenoid Z-isomers with high solubility in SC-CO2. Moreover, the effects of extraction pressure and entrainer content on Z-isomerization and recovery of carotenoids are also investigated. The results show that higher pressure and ethanol content resulted in lower Z-isomer ratios and higher recoveries of carotenoids. As the Z-isomers of carotenoids have greater bioavailability and potentially exhibit superior biological activities than the all-E-isomers, high value-added carotenoids, and higher yield can be simultaneously achieved with high-temperature extraction. Practical applications: As carotenoid extraction is usually performed using toxic organic solvents, residual solvents are a serious concern. In addition, naturally occurring carotenoids (mainly the all-E-isomers) have low bioavailability. These are important issues that need to be overcome for the industrial use of carotenoids. The Z-isomer-rich carotenoids obtained by SC-CO2 extraction under high-temperature conditions have great potential to solve these issues simultaneously.
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页数:9
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