Laboratory diagnosis of human T-lymphotropic virus in Brazil: assays, flowcharts, challenges, and perspectives

被引：16

作者：

Caterino-de-Araujo, Adele ^{[1
]}

Barbosa-Stancioli, Edel Figueiredo ^{[2
]}

Alonso Neto, Jose Boullosa ^{[3
]}

Aragon, Mayra Goncalves ^{[4
]}

Galvao-Castro, Bernardo ^{[5
]}

Ishak, Ricardo ^{[6
]}

Rosadas, Carolina ^{[7
]}

机构：

[1] Adolfo Lutz Inst, Ctr Imunol, Lab Pesquisa HTLV, Sao Paulo, SP, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Lab Virol Basica & Aplicada, Belo Horizonte, MG, Brazil

[3] Minist Saude, Secretaria Vigilancia Saude, Dept Doencas Condicoes Cron & Infeccoes Sexualmen, Brasilia, DF, Brazil

[4] Univ Fed Espirito Santo, Programa Posgrad Doencas Infecciosas, Vitoria, ES, Brazil

[5] Escola Bahiana Med & Saude Publ, Ctr Integrat & Muldisciplinar Atendimento Portado, Salvador, BA, Brazil

[6] Univ Fed Para, Inst Ciencias Biol, Lab Virol, Belem, Para, Brazil

[7] Imperial Coll Healthcare NHS Trust, St Marys Hosp, Natl Ctr Human Retrovirol, London, England

来源：

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL | 2021年 / 54卷

关键词：

Human T-lymphotropic virus 1; Human T-lymphotropic virus 2; Diagnosis; Screening assays; Confirmatory assays; Algorithms; REAL-TIME PCR; HTLV-1 PROVIRAL LOAD; PERIPHERAL-BLOOD; INDIVIDUALS; CARRIERS;

D O I：

10.1590/0037-8682-0175-2021

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Introduction: We present a data analysis and review of recent studies regarding the laboratory diagnosis of human T-lymphotropic virus 1 and 2 (HTLV-1/2) infections in Brazil. Methods: Target populations, available diagnostic serological assays (screening and complementary tests), molecular assays (in-house), causes of false-positive and false-negative results, and flowcharts were analyzed. Results: A table presents the target populations, two diagnostic flowcharts (depending on laboratory infrastructure and study population), and recent research that may improve how HTLV-1/2 is diagnosed in Brazil. Conclusions: Our results support the implementation of public policies to reduce HTLV-1/2 transmission and its associated diseases.

引用

页数：5

共 26 条

[1] Evaluation of the use of real-time PCR for human T cell lymphotropic virus 1 and 2 as a confirmatory test in screening for blood donors [J].

Andrade, Rafaela Gomes ;

Ribeiro, Maisa Aparecida ;

Silva Namen-Lopes, Maria Sueli ;

Nunes Silva, Sonia Mara ;

Basques, Fernando Valadares ;

Ribas, Joao Gabriel ;

de Freitas Carneiro-Proietti, Anna Barbara ;

Martins, Marina Lobato .

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 2010, 43 (02) :111-115

[2] Use of synthetic oligonucleotides for determination of HTLV-1 proviral load by real-time PCR: a helpful alternative approach in the clinical management [J].

Bandeira, L. M. ;

Puga, M. A. M. ;

de Paula, V. S. ;

Demarchi, L. H. F. ;

Lichs, G. G. C. ;

Domingos, J. A. ;

da Cunha, R., V ;

Uehara, S. N. O. ;

Motta-Castro, A. R. C. .

JOURNAL OF APPLIED MICROBIOLOGY, 2020, 129 (03) :768-774

[3] Monitoring the HTLV-1 proviral load in the peripheral blood of asymptomatic carriers and patients with HTLV-associated myelopathy/tropical spastic paraparesis from a Brazilian cohort: ROC curve analysis to establish the threshold for risk disease [J].

Brito Silva Furtado, Marina dos Santos ;

Andrade, Rafaela Gomes ;

Ferreira Romanelli, Luiz Claudio ;

Ribeiro, Maisa Aparecida ;

Ribas, Joao Gabriel ;

Torres, Elidio Barbosa ;

Barbosa-Stancioli, Edel Figueiredo ;

de Freitas Carneiro Proietti, Anna Barbara ;

Martins, Marina Lobato .

JOURNAL OF MEDICAL VIROLOGY, 2012, 84 (04) :664-671

[4] Performance of Commercially Available Serological Screening Tests for Human T-Cell Lymphotropic Virus Infection in Brazil [J].

Brito, Vanessa da Silva ;

Neves Santos, Fred Luciano ;

Sousa Goncalves, Noilson Lazaro ;

Almeida Araujo, Thessika Hialla ;

Vale Nascimento, Davi Silva ;

Pereira, Felicidade Mota ;

Amaral Boa-Sorte, Ney Cristian ;

Rios Grassi, Maria Fernanda ;

Caterino-de-Araujo, Adele ;

Galvao-Castro, Bernardo .

JOURNAL OF CLINICAL MICROBIOLOGY, 2018, 56 (12)

[5] Line Immunoassay for Confirmation and Discrimination of Human T-Cell Lymphotropic Virus Infections in Inconclusive Western Blot Serum Samples from Brazil [J].

Campos, Karoline R. ;

Santos, Fred L. N. ;

Brito, Vanessa da Silva ;

Goncalves, Noilson L. S. ;

Araujo, Thessika H. A. ;

Galvao-Castro, Bernardo ;

Caterino-de-Araujo, Adele .

JOURNAL OF CLINICAL MICROBIOLOGY, 2020, 58 (01)

[6] Provirus Mutations of Human T-Lymphotropic Virus 1 and 2 (HTLV-1 and HTLV-2) in HIV-1-Coinfected Individuals [J].

Campos, Karoline Rodrigues ;

Caterino-de-Araujo, Adele .

MSPHERE, 2020, 5 (05)

[7] Comparative performances of serologic and molecular assays for detecting human T lymphotropic virus type 1 and type 2 (HTLV-1 and HTLV-2) in patients infected with human immunodeficiency virus type 1 (HIV-1) [J].

Campos, Karoline Rodrigues ;

Goncalves, Maria Gisele ;

Costa, Nadia Aparecida ;

Caterino-de-Araujo, Adele .

BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2017, 21 (03) :297-305

[8] The best algorithm to confirm the diagnosis of HTLV-1 and HTLV-2 in at-risk individuals from Sao Paulo, Brazil [J].

Costa, Emanuela A. S. ;

Magri, Mariana C. ;

Caterino-de-Araujo, Adele .

JOURNAL OF VIROLOGICAL METHODS, 2011, 173 (02) :280-286

[9] First Description of Seronegative HTLV-1 Carriers in Argentina [J].

Gallego, Sandra ;

Frutos, Maria C. ;

Blanco, Sebastian ;

Castro, Gonzalo ;

Balangero, Marcos ;

Elias Panigo, David ;

Mangeaud, Arnaldo ;

Remondegui, Carlos ;

Rocha, Anderson Santos ;

Franco, Gabriela Melo ;

Martins, Marina Lobato ;

Barbosa-Stancioli, Edel Figueiredo ;

Nates, Silvia .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2020, 102 (04) :889-895

[10] Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection [J].

Gomes, Yago ;

Caterino-de-Araujo, Adele ;

Campos, Karoline ;

Goncalves, Maria Gisele ;

Leite, Ana Claudia ;

Lima, Marco Antonio ;

Araujo, Abelardo ;

Silva, Marcus Tulius ;

Espindola, Otavio .

VIRUSES-BASEL, 2020, 12 (09)

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