Detection of HCV and GBV-C/HGV infection by multiplex PCR in plasma samples of transfused subjects

被引：9

作者：

Caudai, C ^{[1
]}

Padula, MG ^{[1
]}

Bettini, V ^{[1
]}

Valensin, PE ^{[1
]}

机构：

[1] Univ Siena, Dept Mol Biol, Microbiol Sect, I-53100 Siena, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 70卷 / 01期

关键词：

GBV-C/HGV; hepatitis C virus; Co-infection; PCR;

D O I：

10.1016/S0166-0934(97)00171-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A multiplex polymerase chain reaction (PCR) was applied to clinical samples for simultaneous detection of hepatitis C virus (HCV) and GBV-C/HGV genome. With both RNA viruses, the amplification was performed with primers of the 5' UTR region starting from the single viral RNA reverse transcripted (cDNA) with random hexanucleotide primer mix. GBV-C/HGV RNA was detected in plasma sample of seven out of 50 transfused patients (14%). The multiplex PCR demonstrated a sensitivity up to 7.8 x 10(2) copies/ml respectively for GBV-C/HGV and HCV RNA in plasma samples of 5/50 patients with GBV-C/HGV/HCV co-infection and in patients with HCV (27/50) or GBV-C/HGV infection alone (2/50). (C) 1998 Elsevier Science B.V.

引用

页码：79 / 83

页数：5