Identification of a Killer Toxin from Wickerhamomyces anomalus with β-Glucanase Activity

被引：17

作者：

Cecarini, Valentina ^{[1
]}

Cuccioloni, Massimiliano ^{[1
]}

Bonfili, Laura ^{[1
]}

Ricciutelli, Massimo ^{[2
]}

Valzano, Matteo ^{[1
]}

Cappelli, Alessia ^{[1
]}

Amantini, Consuelo ^{[1
]}

Favia, Guido ^{[1
]}

Eleuteri, Anna Maria ^{[1
]}

Angeletti, Mauro ^{[1
]}

Ricci, Irene ^{[1
]}

机构：

[1] Univ Camerino, Sch Biosci & Vet Med, I-62032 Camerino, Italy

[2] Univ Camerino, HPLC MS Lab, I-62032 Camerino, Italy

来源：

TOXINS | 2019年 / 11卷 / 10期

基金：

欧洲研究理事会;

关键词：

Wickerhamomyces anomalus; killer toxin; malaria; symbiotic control; PICHIA-ANOMALA; YEAST; GLUCOSIDASE; PEPTIDES; SYSTEM;

D O I：

10.3390/toxins11100568

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The yeast Wickerhamomyces anomalus has several applications in the food industry due to its antimicrobial potential and wide range of biotechnological properties. In particular, a specific strain of Wickerhamomyces anomalus isolated from the malaria mosquito Anopheles stephensi, namely WaF17.12, was reported to secrete a killer toxin with strong anti-plasmodial effect on different developmental stages of Plasmodium berghei; therefore, we propose its use in the symbiotic control of malaria. In this study, we focused on the identification/characterization of the protein toxin responsible for the observed antimicrobial activity of the yeast. For this purpose, the culture medium of the killer yeast strain WaF17.12 was processed by means of lateral flow filtration, anion exchange and gel filtration chromatography, immunometric methods, and eventually analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Based on this concerted approach, we identified a protein with a molecular weight of approximately 140 kDa and limited electrophoretic mobility, corresponding to a high molecular weight beta-glucosidase, as confirmed by activity tests in the presence of specific inhibitors.

引用

页数：11

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