Putting the Spotlight Back on Plant Suspension Cultures

被引:125
作者
Santos, Rita B. [1 ]
Abranches, Rita [1 ]
Fischer, Rainer [2 ,3 ]
Sack, Markus [3 ]
Holland, Tanja [2 ]
机构
[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Plant Cell Biol Lab, P-2780156 Oeiras, Portugal
[2] Fraunhofer Inst Mol Biol & Angew Oekol IME, Integrated Prod Platforms, Aachen, Germany
[3] Rhein Westfal TH Aachen, Inst Mol Biotechnol, Biol 7, Aachen, Germany
关键词
plant suspension cultures; biopharmaceuticals; BY-2; protein production; plant cell cultures; COLONY-STIMULATING FACTOR; HUMAN SERUM-ALBUMIN; RECOMBINANT PROTEIN-PRODUCTION; ENZYME REPLACEMENT THERAPY; HIGH-YIELD PRODUCTION; HUMAN GROWTH-HORMONE; MEDICAGO-TRUNCATULA; CELL-CULTURES; PHARMACEUTICAL PROTEINS; MONOCLONAL-ANTIBODY;
D O I
10.3389/fpls.2016.00297
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant cell suspension cultures have several advantages that make them suitable for the production of recombinant proteins. They can be cultivated under aseptic conditions using classical fermentation technology, they are easy to scale-up for manufacturing, and the regulatory requirements are similar to those established for well-characterized production systems based on microbial and mammalian cells. It is therefore no surprise that taliglucerase alfa (Elelyso (R))-the first licensed recombinant pharmaceutical protein derived from plants-is produced in plant cell suspension cultures. But despite this breakthrough, plant cells are still largely neglected compared to transgenic plants and the more recent plant-based transient expression systems. Here, we revisit plant cell suspension cultures and highlight recent developments in the field that show how the rise of plant cells parallels that of Chinese hamster ovary cells, currently the most widespread and successful manufacturing platform for biologics. These developments include medium optimization, process engineering, statistical experimental designs, scale-up/scale-down models, and process analytical technologies. Significant yield increases for diverse target proteins will encourage a gold rush to adopt plant cells as a platform technology, and the first indications of this breakthrough are already on the horizon.
引用
收藏
页数:12
相关论文
共 136 条
[1]   Plants as bioreactors:: A comparative study suggests that Medicago truncatula is a promising production system [J].
Abranches, R ;
Marcel, S ;
Arcalis, E ;
Altmann, F ;
Fevereiro, P ;
Stoger, E .
JOURNAL OF BIOTECHNOLOGY, 2005, 120 (01) :121-134
[2]   An efficient transformation method to regenerate a high number of transgenic plants using a new embryogenic line of Medicago truncatula cv. Jemalong [J].
Araújo, SD ;
Duque, ASRLA ;
dos Santos, DMMF ;
Fevereiro, MPS .
PLANT CELL TISSUE AND ORGAN CULTURE, 2004, 78 (02) :123-131
[3]   Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins [J].
Arfi, Zulfaquar Ahmad ;
Hellwig, Stephan ;
Drossard, Jurgen ;
Fischer, Rainer ;
Buyel, Johannes Felix .
BIOTECHNOLOGY JOURNAL, 2016, 11 (04) :507-518
[4]   Plant-made pharmaceuticals: from 'Edible Vaccines' to Ebola therapeutics [J].
Arntzen, Charles .
PLANT BIOTECHNOLOGY JOURNAL, 2015, 13 (08) :1013-1016
[5]   Recombinant protein folding and misfolding in Escherichia coli [J].
Baneyx, F ;
Mujacic, M .
NATURE BIOTECHNOLOGY, 2004, 22 (11) :1399-1408
[6]   Enhanced recovery of a secreted recombinant human growth factor using stabilizing additives and by co-expression of human serum albumin in the moss Physcomitrella patens [J].
Baur, A ;
Reski, R ;
Gorr, G .
PLANT BIOTECHNOLOGY JOURNAL, 2005, 3 (03) :331-340
[7]  
Benchabane Meriem, 2009, V483, P265, DOI 10.1007/978-1-59745-407-0_15
[8]  
Bonnart R, 2010, CRYOLETTERS, V31, P200
[9]   N-Glycosylation of Plant-produced Recombinant Proteins [J].
Bosch, Dirk ;
Castilho, Alexandra ;
Loos, Andreas ;
Schots, Arjen ;
Steinkellner, Herta .
CURRENT PHARMACEUTICAL DESIGN, 2013, 19 (31) :5503-5512
[10]   Metabolic profiling of Medicago truncatula cell cultures reveals the effects of biotic and abiotic elicitors on metabolism [J].
Broeckling, CD ;
Huhman, DV ;
Farag, MA ;
Smith, JT ;
May, GD ;
Mendes, P ;
Dixon, RA ;
Sumner, LW .
JOURNAL OF EXPERIMENTAL BOTANY, 2005, 56 (410) :323-336