The Gram-negative plant pathogen Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. The production of Xcc virulence factors is regulated by Clp and RpfF. HD-related output domain (HDOD) is a protein domain of unknown biochemical function. The genome of Xcc encodes three proteins (GsmR, HdpA, and HdpB) with an HDOD. The GsmR has been reported to play a role in the general stress response and cell motility and its expression is positively regulated by Clp. Here, the function and transcription of hdpA and hdpB were characterized. Mutation of hdpA resulted in enhanced bacterial attachment. In addition, the expression of hdpA was positively regulated by RpfF but not by Clp, subject to catabolite repression and affected by several stress conditions. However, mutational analysis and reporter assay showed that hdpB had no effect on the production of a range of virulence factors and its expression was independent of Clp and RpfF. The results shown here not only extend the previous work on RpfF regulation to show that it influences the expression of hdpA in Xcc, but also expand knowledge of the function of the HDOD containing proteins in bacteria.
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HHMI Janelia Farm Res Campus, Ashburn, VA 20147 USAHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Finn, Robert D.
Bateman, Alex
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European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Bateman, Alex
Clements, Jody
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HHMI Janelia Farm Res Campus, Ashburn, VA 20147 USAHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Clements, Jody
Coggill, Penelope
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European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, England
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Coggill, Penelope
Eberhardt, Ruth Y.
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European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, England
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Eberhardt, Ruth Y.
Eddy, Sean R.
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HHMI Janelia Farm Res Campus, Ashburn, VA 20147 USAHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Eddy, Sean R.
Heger, Andreas
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Univ Oxford, MRC Funct Genom Unit, Dept Physiol Anat & Genet, Oxford OX1 3QX, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Heger, Andreas
Hetherington, Kirstie
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Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Hetherington, Kirstie
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Holm, Liisa
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Mistry, Jaina
Sonnhammer, Erik L. L.
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Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, Stockholm Bioinformat Ctr,Swedish eSci Res Ctr, SE-17121 Solna, SwedenHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Sonnhammer, Erik L. L.
Tate, John
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European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, England
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
机构:
HHMI Janelia Farm Res Campus, Ashburn, VA 20147 USAHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Finn, Robert D.
Bateman, Alex
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机构:
European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Bateman, Alex
Clements, Jody
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HHMI Janelia Farm Res Campus, Ashburn, VA 20147 USAHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Clements, Jody
Coggill, Penelope
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h-index: 0
机构:
European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, England
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Coggill, Penelope
Eberhardt, Ruth Y.
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h-index: 0
机构:
European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, England
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Eberhardt, Ruth Y.
Eddy, Sean R.
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h-index: 0
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HHMI Janelia Farm Res Campus, Ashburn, VA 20147 USAHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Eddy, Sean R.
Heger, Andreas
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h-index: 0
机构:
Univ Oxford, MRC Funct Genom Unit, Dept Physiol Anat & Genet, Oxford OX1 3QX, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Heger, Andreas
Hetherington, Kirstie
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h-index: 0
机构:
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Hetherington, Kirstie
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Holm, Liisa
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Mistry, Jaina
Sonnhammer, Erik L. L.
论文数: 0引用数: 0
h-index: 0
机构:
Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, Stockholm Bioinformat Ctr,Swedish eSci Res Ctr, SE-17121 Solna, SwedenHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA
Sonnhammer, Erik L. L.
Tate, John
论文数: 0引用数: 0
h-index: 0
机构:
European Bioinformat Inst EMBL EBI, European Mol Biol Lab, Cambridge CB10 1SD, England
Wellcome Trust Sanger Inst, Cambridge CB10 1SA, EnglandHHMI Janelia Farm Res Campus, Ashburn, VA 20147 USA