Rapid determination of some psychotropic drugs in complex matrices by tandem dispersive liquid-liquid microextraction followed by high performance liquid chromatography

被引：27

作者：

Asghari, Alireza ^{[1
]}

Fahimi, Ebrahim ^{[1
]}

Bazregar, Mohammad ^{[1
]}

Rajabi, Maryam ^{[1
]}

Boutorabi, Leila ^{[1
]}

机构：

[1] Semnan Univ, Dept Chem, Semnan 2333383193, Iran

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2017年 / 1052卷

关键词：

Tandem dispersive liquid-liquid microextraction; Psychotropic drugs; High performance liquid chromatography-ultraviolet detection; SOLID-PHASE EXTRACTION; SAMPLE PREPARATION; ANTIPSYCHOTIC-DRUGS; BIOLOGICAL-FLUIDS; HOLLOW-FIBER; SOLVENT MICROEXTRACTION; COMPLICATED MATRICES; EFFICIENT METHOD; TRACE AMOUNTS; HUMAN PLASMA;

D O I：

10.1016/j.jchromb.2017.03.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simple and rapid determinations of some psychotropic drugs in some pharmaceutical wastewater and human plasma samples were successfully accomplished via the tandem dispersive liquid-liquid microextraction combined with high performance liquid chromatography-ultraviolet detection (TDLLME-HPLC-UV). TDLLME of the three psychotropic drugs clozapine, chlorpromazine, and thioridazine was easily performed through two consecutive dispersive liquid-liquid microextractions. By performing this convenient method, proper sample preconcentrations and clean-ups were achieved in just about 7 min. In order to achieve the best extraction efficiency, the effective parameters involved were optimized. The optimal experimental conditions consisted of 100 mu L of CCl4 (as the extraction organic solvent), and the pH values of 13 and 2 for the donor and acceptor phases, respectively. Under these optimum experimental conditions, the proposed TDLLME-HPLC-UV technique provided a good linearity in the range of 5-3000 ng mL(-1) for the three psychotropic drugs with the correlation of determinations (R(2)s) higher than 0.996. The limits of quantification (LOQs) and limits of detection (LODs) obtained were 5.0 ngmL(-1) and 1.0-1.5 ngmL(-1), respectively. Also the proper enrichment factors (EFs) of 96, 99, and 88 for clozapine, chlorpromazine, and thioridazine, respectively, and good extraction repeatabilities (relative standard deviations below 9.3%, n = 5) were obtained. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：51 / 59

页数：9

共 45 条

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