Glycoprotein Expression in Human Milk during Lactation

被引：75

作者：

Froehlich, John W. ^{[1
]}

Dodds, Eric D. ^{[1
]}

Barboza, Mariana ^{[1
]}

McJimpsey, Erica L. ^{[1
]}

Seipert, Richard R. ^{[1
]}

Francis, Jimi ^{[4
]}

An, Hyun Joo ^{[1
]}

Freeman, Samara ^{[2
]}

German, J. Bruce ^{[2
]}

Lebrilla, Carlito B. ^{[1
,3
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA

[3] Univ Calif Davis, Sch Med, Davis, CA 95616 USA

[4] Univ Nevada, Dept Biochem, Reno, NV 89557 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 10期

关键词：

Breast milk; glycoprotein; lactation; milk; post-translational modification; SALT-STIMULATED LIPASE; HIGH-MASS ACCURACY; PHYSIOLOGICAL SIGNIFICANCE; PROTEIN IDENTIFICATION; STATISTICAL-MODEL; HUMAN COLOSTRUM; PEPTIDE MASS; GLYCOSYLATION; LACTOFERRIN; OLIGOSACCHARIDES;

D O I：

10.1021/jf100112x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

While milk proteins have been studied for decades, strikingly little effort has been applied to determining how the post-translational modifications (PTMs) of these proteins may change during the course of lactation. PTMs, particularly glycosylation, can greatly influence protein structure, function, and stability and can particularly influence the gut where their degradation products are potentially bioactive. In this work, previously undiscovered temporal variations in both expression and glycosylation of the glycoproteome of human milk are observed. Lactoferrin, one of the most abundant glycoproteins in human milk, is shown to be dynamically glycosylated during the first 10 days of lactation. Variations in expression or glycosylation levels are also demonstrated for several other abundant whey proteins, including tenascin, bile salt-stimulated lipase, xanthine dehydrogenase, and mannose receptor.

引用

页码：6440 / 6448

页数：9

共 51 条

[1] On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database [J].