Accelerated Neuritogenesis and Maturation of Primary Spinal Motor Neurons in Response to Nanofibers

被引:75
作者
Gertz, Caitlyn C. [1 ]
Leach, Michelle K. [1 ,2 ,3 ]
Birrell, Lisa K. [1 ]
Martin, David C. [2 ,4 ]
Feldman, Eva L. [1 ]
Corey, Joseph M. [1 ,2 ,3 ]
机构
[1] Univ Michigan, Dept Neurol, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[3] VA Ann Arbor Healthcare Ctr, Ctr Geriatr Res Educ & Clin, Ann Arbor, MI 48105 USA
[4] Univ Michigan, Dept Mat Sci & Engn, Ann Arbor, MI 48109 USA
关键词
motor neuron; neuritogenesis; neuronal polarity; nanofibers; guidance; lamellipdodia; CULTURED HIPPOCAMPAL-NEURONS; ELECTROSPUN NANOFIBERS; IN-VITRO; MICROPATTERNED SURFACES; LOCAL PRESENTATION; CONTACT GUIDANCE; NEURITE GROWTH; N-CADHERIN; POLARITY; SCAFFOLDS;
D O I
10.1002/dneu.20792
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Neuritogenesis, neuronal polarity formation, and maturation of axons and dendrites are strongly influenced by both biochemical and topographical extracellular components. The aim of this study was to elucidate the effects of polylactic acid electrospun fiber topography on primary motor neuron development, because regeneration of motor axons is extremely limited in the central nervous system and could potentially benefit from the implementation of a synthetic scaffold to encourage regrowth. In this analysis, we found that both aligned and randomly oriented submicron fibers significantly accelerated the processes of neuritogenesis and polarity formation of individual cultured motor neurons compared to flat polymer films and glass controls, likely due to restricted lamellipodia formation observed on fibers. In contrast, dendritic Maturation and soma spreading were inhibited on fiber substrates after 2 days in vitro. This study is the first to examine the effects of electrospun fiber topography on motor neuron neuritogenesis and polarity formation. Aligned nanofibers were shown to affect the directionality and timing of motor neuron development, providing further evidence for the effective use of electrospun scaffolds in neural regeneration applications. (C) 2010 Wiley Periodicals. Inc. Develop Neurobiol 70: 589-603, 2010
引用
收藏
页码:589 / 603
页数:15
相关论文
共 56 条
[1]  
Andersen SSL, 2000, BIOESSAYS, V22, P172, DOI 10.1002/(SICI)1521-1878(200002)22:2<172::AID-BIES8>3.0.CO
[2]  
2-Q
[3]   Key regulators in neuronal polarity [J].
Arimura, N ;
Kaibuchi, K .
NEURON, 2005, 48 (06) :881-884
[4]   Neuronal polarity: from extracellular signals to intracellular mechanisms [J].
Arimura, Nariko ;
Kaibuchi, Kozo .
NATURE REVIEWS NEUROSCIENCE, 2007, 8 (03) :194-205
[5]   Pars, PI 3-kinase, and the establishment of neuronal polarity [J].
Banker, G .
CELL, 2003, 112 (01) :4-5
[6]   AXONAL BRANCHING AND GROWTH CONE STRUCTURE DEPEND ON TARGET-CELLS [J].
BERMAN, SA ;
MOSS, D ;
BURSZTAJN, S .
DEVELOPMENTAL BIOLOGY, 1993, 159 (01) :153-162
[7]   OPTIMIZED SURVIVAL OF HIPPOCAMPAL-NEURONS IN B27-SUPPLEMENTED NEUROBASAL(TM), A NEW SERUM-FREE MEDIUM COMBINATION [J].
BREWER, GJ ;
TORRICELLI, JR ;
EVEGE, EK ;
PRICE, PJ .
JOURNAL OF NEUROSCIENCE RESEARCH, 1993, 35 (05) :567-576
[8]   MICROPATTERNED SUBSTRATUM ADHESIVENESS - A MODEL FOR MORPHOGENETIC CUES CONTROLLING CELL BEHAVIOR [J].
BRITLAND, S ;
CLARK, P ;
CONNOLLY, P ;
MOORES, G .
EXPERIMENTAL CELL RESEARCH, 1992, 198 (01) :124-129
[9]   SUPPRESSION OF MAP2 IN CULTURED CEREBELLAR MACRONEURONS INHIBITS MINOR NEURITE FORMATION [J].
CACERES, A ;
MAUTINO, J ;
KOSIK, KS .
NEURON, 1992, 9 (04) :607-618
[10]   Aligned protein-polymer composite fibers enhance nerve regeneration: A potential tissue-engineering platform [J].
Chew, Sing Yian ;
Mi, Ruifa ;
Hoke, Ahmet ;
Leong, Kam W. .
ADVANCED FUNCTIONAL MATERIALS, 2007, 17 (08) :1288-1296