Simultaneous untargeted and targeted metabolomics profiling of underivatized primary metabolites in sulfur-deficient barley by ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry

被引:43
作者
Ghosson, Hikmat [1 ,2 ]
Schwarzenberg, Adrian [1 ]
Jamois, Frank [1 ]
Yvin, Jean-Claude [1 ]
机构
[1] Ctr Mondial Innovat Roullier CMI, 18 Ave Franklin Roosevelt, F-35400 St Malo, France
[2] Univ Rennes 1, UFR Sci & Proprietes Matiere, 2 Rue Thabor,CS 46510, F-35065 Rennes, France
关键词
Plant metabolomics; Primary metabolites; Organic acids; Amino acids; LC-HRMS; Sulfur deficiency; FREE AMINO-ACIDS; ARABIDOPSIS-THALIANA; OXIDIZED GLUTATHIONE; PLANT-TISSUES; MAIZE HYBRIDS; LC-MS/MS; RESPONSES; MS; CYSTEINE; EXTRACTS;
D O I
10.1186/s13007-018-0329-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: Metabolomics based on mass spectrometry analysis are increasingly applied in diverse scientific domains, notably agronomy and plant biology, in order to understand plants' behaviors under different stress conditions. In fact, these stress conditions are able to disrupt many biosynthetic pathways that include mainly primary metabolites. Profiling and quantifying primary metabolites remain a challenging task because they are poorly retained in reverse phase columns, due to their high polarity and acid-base properties. The aim of this work is to develop a simultaneous untargeted/targeted profiling of amino acids, organic acids, sulfur metabolites, and other several metabolites. This method will be applied on sulfur depleted barley, in order to study this type of stress, which is difficult to detect at early stage. Also, this method aims to explore the impact of this stress on barley's metabolome. Results: Ultra-high performance liquid chromatography-high resolution mass spectrometry-based method was successfully applied to real samples allowing to discriminate, detect, and quantify primary metabolites in short-runs without any additional sampling step such as derivatization or ion pairing. The retention of polar metabolites was successfully achieved using modified C18 columns with high reproducibility (relative standard deviation below 10%). The quantification method showed a high sensitivity and robustness. Furthermore, high resolution mass spectrometry detection provided reliable quantification based on exact mass, eliminating potential interferences, and allowing the simultaneous untargeted metabolomics analysis. The untargeted data analysis was conducted using Progenesis QI software, performing alignment, peak picking, normalization and multivariate analysis. The simultaneous analysis provided cumulative information allowing to discriminate between two plant batches. Thus, discriminant biomarkers were identified and validated. Simultaneously, quantification confirmed coherently the relative abundance of these biomarkers. Conclusions: A fast and innovated simultaneous untargeted/targeted method has successfully been developed and applied to sulfur deficiency on barley. This work opens interesting perspectives in both fundamental and applied research. Biomarker discovery give precious indication to understand plant behavior during a nutritional deficiency. Thus, direct or indirect measurement of these compounds allows a real time fertilization management and encounter the challenges of sustainable agriculture.
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页数:17
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共 48 条
[1]   Use of reverse-phase liquid chromatography, linked to tandem mass spectrometry, to profile the Calvin cycle and other metabolic intermediates in Arabidopsis rosettes at different carbon dioxide concentrations [J].
Arrivault, Stephanie ;
Guenther, Manuela ;
Ivakov, Alexander ;
Feil, Regina ;
Vosloh, Daniel ;
van Dongen, Joost T. ;
Sulpice, Ronan ;
Stitt, Mark .
PLANT JOURNAL, 2009, 59 (05) :824-839
[2]   Effect of Environment and Genotype on Commercial Maize Hybrids Using LC/MS-Based Metabolomics [J].
Baniasadi, Hamid ;
Vlahakis, Chris ;
Hazebroek, Jan ;
Zhong, Cathy ;
Asiago, Vincent .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (06) :1412-1422
[3]   Current and emerging mass-spectrometry technologies for metabolomics [J].
Bedair, Mohamed ;
Sumner, Lloyd W. .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2008, 27 (03) :238-250
[4]   Simultaneous determination of reduced and oxidized glutathione in peripheral blood mononuclear cells by liquid chromatography-electro spray mass spectrometry [J].
Camera, E ;
Rinaldi, M ;
Briganti, S ;
Picardo, M ;
Fanali, S .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2001, 757 (01) :69-78
[5]   Nitrogen assimilation and cysteine biosynthesis in barley: Evidence for root sulphur assimilation upon recovery from N deprivation [J].
Carfagna, Simona ;
Vona, Vincenza ;
Di Martino, Vittoria ;
Esposito, Sergio ;
Rigano, Carmelo .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2011, 71 (01) :18-24
[6]   Plant secondary metabolite profiling evidences strain-dependent effect in the Azospirillum-Oryza sativa association [J].
Chamam, Amel ;
Sanguin, Herve ;
Bellvert, Floriant ;
Meiffren, Guillaume ;
Comte, Gilles ;
Wisniewski-Dye, Florence ;
Bertrand, Cedric ;
Prigent-Combaret, Claire .
PHYTOCHEMISTRY, 2013, 87 :65-77
[7]   Modified method for determination of sulfur metabolites in plant tissues by stable isotope dilution-based liquid chromatography-electrospray ionization-tandem mass spectrometry [J].
Chang, Ya-Lan ;
Hsieh, Chin-Lin ;
Huang, Yao-Moan ;
Chiou, Wen-Liang ;
Kuo, Yueh-Hsiung ;
Tseng, Mei-Hwei .
ANALYTICAL BIOCHEMISTRY, 2013, 442 (01) :24-33
[8]   Fumaric acid:: an overlooked form of fixed carbon in Arabidopsis and other plant species [J].
Chia, DW ;
Yoder, TJ ;
Reiter, WD ;
Gibson, SI .
PLANTA, 2000, 211 (05) :743-751
[9]   Hydrophilic interaction LC combined with electrospray MS for highly sensitive analysis of underivatized amino acids in rhizosphere research [J].
Dell'mour, Madeleine ;
Jaitz, Leonhard ;
Oburger, Eva ;
Puschenreiter, Markus ;
Koellensperger, Gunda ;
Hann, Stephan .
JOURNAL OF SEPARATION SCIENCE, 2010, 33 (6-7) :911-922
[10]   Identification of metabolites associated with water stress responses in Solanum tuberosum L. clones [J].
Drapal, M. ;
Farfan-Vignolo, E. R. ;
Gutierrez, O. R. ;
Bonierbale, M. ;
Mihovilovich, E. ;
Fraser, P. D. .
PHYTOCHEMISTRY, 2017, 135 :24-33