Studies of P(L/D)LA 96/4 non-woven scaffolds and fibres;: properties, wettability and cell spreading before and after intrusive treatment methods

被引:12
作者
Ella, Ville
Gomes, Manuela E.
Reis, Rui L.
Tormala, Pertti
Kellomaki, Minna
机构
[1] Tampere Univ Technol, Inst Biomat, FIN-33101 Tampere, Finland
[2] Univ Minho, Res Grp Biomat Biodegradables & Biomimet, P-4710057 Braga, Portugal
[3] Univ Minho, Dept Polymer Engn, P-4800058 Guimaraes, Portugal
关键词
D O I
10.1007/s10856-007-0144-z
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Poly(L/D)lactide 96/4 fibres with diameters of 50 and 80 mu m were produced. The smaller diameter fibres were carded and needle punched to form a non-woven mat. Fibres and non-woven mats were hydrolysed for a period of 20 weeks. Fibres and pressed non-woven discs were treated with low-temperature oxygen plasma and alkaline KOH hydrolysis and ethanol washing was used as a reference treatment. The non-wovens lost 50% of their tear strength after 8 weeks in vitro while the fibres still retained 65% tensile strength after 20 weeks. Hydrolysation time in KOH, treatment time and power settings of the oxygen plasma were all directly proportional to the mechanical properties of the fibres. Increasing time (and power) resulted in lower tensile properties. Rapid wetting of the scaffolds was achieved by oxygen plasma, KOH hydrolysation and ethanol washing. Cell culturing using fibroblast cell line was carried out for the treated and non-treated non-woven scaffolds. In terms of adhesion and the spreading of the cells into the scaffold, best results after 3-day culturing were obtained for the oxygen plasma treated scaffolds.
引用
收藏
页码:1253 / 1261
页数:9
相关论文
共 25 条
[21]   Adsorption of albumin, collagen, and fibronectin on the surface of poly(hydroxybutyrate-hydroxyvalerate) (PHB/HV) and of poly(ε-caprolactone) (PCL) films modified by an alkaline hydrolysis and of poly(ethylene terephtalate) (PET) track-etched membranes [J].
Rouxhet, L ;
Duhoux, F ;
Borecky, O ;
Legras, R ;
Schneider, YJ .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 1998, 9 (12) :1279-1304
[22]  
Sittinger M, 1996, J BIOMED MATER RES, V33, P57, DOI 10.1002/(SICI)1097-4636(199622)33:2<57::AID-JBM1>3.0.CO
[23]  
2-K
[24]   Cell adhesion on gaseous plasma modified poly-(L-lactide) surface under shear stress field [J].
Wan, YQ ;
Yang, J ;
Yang, JL ;
Bei, JZ ;
Wang, SG .
BIOMATERIALS, 2003, 24 (21) :3757-3764
[25]   Enhancing the cell affinity of macroporous poly(L-lactide) cell scaffold by a convenient surface modification method [J].
Yang, J ;
Wan, YQ ;
Tu, CF ;
Cai, Q ;
Bei, JZ ;
Wang, SG .
POLYMER INTERNATIONAL, 2003, 52 (12) :1892-1899