Crystal structure of translation initiation factor 5B from the crenarchaeon Aeropyrum pernix

被引：6
作者：
Murakami, Ryo [1 ]
Miyoshi, Tomohiro [2 ]
Uchiumi, Toshio [1 ,3 ]
Ito, Kosuke [1 ,3 ]
机构：
[1] Niigata Univ, Grad Sch Sci & Technol, Dept Food & Life Sci, Niigata 9502181, Japan
[2] Niigata Univ, Ctr Transdisciplinary Res, Niigata 9502181, Japan
[3] Niigata Univ, Dept Biol, Fac Sci, Niigata 9502181, Japan
来源：
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2016年 / 84卷 / 05期
基金：
日本学术振兴会;
关键词：
translational GTPase; GTP hydrolysis; ribosomal subunit joining; X-ray crystallography; molecular evolution; X-RAY STRUCTURES; SULFOLOBUS-SOLFATARICUS; RIBOSOMAL-SUBUNIT; ESCHERICHIA-COLI; DIMENSIONS; FACTORS; 1A; COMPLEX; BINDING; EIF5B; EIF1A;
D O I：
10.1002/prot.25009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Initiation factor 5B (IF5B) is a universally conserved translational GTPase that catalyzes ribosomal subunit joining. In eukaryotes, IF5B directly interacts via a groove in its domain IV with initiation factor 1A (IF1A), another universally conserved initiation factor, to accomplish efficient subunit joining. Here, we have determined the first structure of a crenarchaeal IF5B, which revealed that the archaea-specific region of IF5B (helix 15) binds and occludes the groove of domain IV. Therefore, archaeal IF5B cannot access IF1A in the same manner as eukaryotic IF5B. This fact suggests that different relationships between IF5B and IF1A exist in archaea and eukaryotes. Proteins 2016; 84:712-717. (c) 2016 Wiley Periodicals, Inc.
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收藏
页码：712 / 717
页数：6
相关论文
共 37 条
  • [1] Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining
    Acker, MG
    Shin, BS
    Dever, TE
    Lorsch, JR
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (13) : 8469 - 8475
  • [2] 3-DIMENSIONAL STRUCTURE OF THE RIBOSOMAL TRANSLOCASE - ELONGATION-FACTOR-G FROM THERMUS-THERMOPHILUS
    AEVARSSON, A
    BRAZHNIKOV, E
    GARBER, M
    ZHELTONOSOVA, J
    CHIRGADZE, Y
    AL-KARADAGHI, S
    SVENSSON, LA
    LILJAS, A
    [J]. EMBO JOURNAL, 1994, 13 (16) : 3669 - 3677
  • [3] Development and characterization of a reconstituted yeast translation initiation system
    Algire, MA
    Maag, D
    Savio, P
    Acker, MG
    Tarun, SZ
    Sachs, AB
    Asano, K
    Nielsen, KH
    Olsen, DS
    Phan, L
    Hinnebusch, AG
    Lorsch, JR
    [J]. RNA, 2002, 8 (03) : 382 - 397
  • [4] BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0
  • [5] Function of eukaryotic translation initiation factor 1A (eIF1A) (formerly called eIF-4C) in initiation of protein synthesis
    Chaudhuri, J
    Si, K
    Maitra, U
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (12) : 7883 - 7891
  • [6] Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2
    Choi, SK
    Olsen, DS
    Roll-Mecak, A
    Martung, A
    Remo, KL
    Burley, SK
    Hinnebusch, AG
    Dever, TE
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (19) : 7183 - 7191
  • [7] Coot:: model-building tools for molecular graphics
    Emsley, P
    Cowtan, K
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 2126 - 2132
  • [8] Molecular Architecture of a Eukaryotic Translational Initiation Complex
    Fernandez, Israel S.
    Bai, Xiao-Chen
    Hussain, Tanweer
    Kelley, Ann C.
    Lorsch, Jon R.
    Ramakrishnan, V.
    Scheres, Sjors H. W.
    [J]. SCIENCE, 2013, 342 (6160) : 824 - +
  • [9] Coupled release of eukaryotic translation initiation factors 5B and 1A from 80S ribosomes following subunit joining
    Fringer, Jeanne M.
    Acker, Michael G.
    Fekete, Christie A.
    Lorsch, Jon R.
    Dever, Thomas E.
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (06) : 2384 - 2397
  • [10] The archaeal 'TACK' superphylum and the origin of eukaryotes
    Guy, Lionel
    Ettema, Thijs J. G.
    [J]. TRENDS IN MICROBIOLOGY, 2011, 19 (12) : 580 - 587
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