Expression of G-Protein Coupled Receptors in Escherichia coli for Structural Studies

被引：28

作者：

Petrovskaya, L. E. ^{[1
]}

Shulga, A. A. ^{[1
]}

Bocharova, O. V. ^{[1
]}

Ermolyuk, Ya S. ^{[1
]}

Kryukova, E. A. ^{[1
]}

Chupin, V. V. ^{[1
]}

Blommers, M. J. J. ^{[2
]}

Arseniev, A. S. ^{[1
]}

Kirpichnikov, M. P. ^{[1
,3
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Novartis Inst BioMed Res, Basel, Switzerland

[3] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

autoinduction; expression in E. coli; GPCR; OmpF; Mistic; MEMBRANE-PROTEINS; CRYSTAL-STRUCTURE; NMR-SPECTROSCOPY; LIGAND-BINDING; E; COLI; PURIFICATION; GPCR; SOLUBILIZATION;

D O I：

10.1134/S0006297910070102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elaborate a high-performance system for expression of genes of G-protein coupled receptors (GPCR), methods of direct and hybrid expression of 17 GPCR genes in Escherichia coli and selection of strains and bacteria cultivation conditions were investigated. It was established that expression of most of the target GPCR fused with the N-terminal fragment of OmpF or Mistic using media for autoinduction provides high output (up to 50 mg/liter).

引用

页码：881 / 891

页数：11