Myofibroblast-Derived SFRP1 as Potential Inhibitor of Colorectal Carcinoma Field Effect

被引：35

作者：

Valcz, Gabor ^{[1
]}

Patai, Arpad V. ^{[2
]}

Kalmar, Alexandra ^{[2
]}

Peterfia, Balint ^{[2
]}

Furi, Istvan ^{[2
]}

Wichmann, Barnabas ^{[1
]}

Muzes, Gyoergyi ^{[2
]}

Sipos, Ferenc ^{[2
]}

Krenacs, Tibor ^{[3
]}

Mihaly, Emese ^{[2
]}

Spisak, Sandor ^{[4
]}

Molnar, Bela ^{[1
,2
]}

Tulassay, Zsolt ^{[1
,2
]}

机构：

[1] Hungarian Acad Sci, Mol Med Res Unit, Budapest, Hungary

[2] Semmelweis Univ, Dept Internal Med 2, H-1085 Budapest, Hungary

[3] Semmelweis Univ, Dept Pathol & Expt Canc Res 1, H-1085 Budapest, Hungary

[4] Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA

来源：

PLOS ONE | 2014年 / 9卷 / 11期

关键词：

FRIZZLED-RELATED PROTEIN-1; WNT SIGNALING PATHWAY; BETA-CATENIN; COLON-CANCER; PROMOTER METHYLATION; WNT/BETA-CATENIN; GENES; EXPRESSION; CELLS; BINDS;

D O I：

10.1371/journal.pone.0106143

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Epigenetic changes of stromal-epithelial interactions are of key importance in the regulation of colorectal carcinoma (CRC) cells and morphologically normal, but genetically and epigenetically altered epithelium in normal adjacent tumor (NAT) areas. Here we demonstrated retained protein expression of well-known Wnt inhibitor, secreted frizzled-related protein 1 (SFRP1) in stromal myofibroblasts and decreasing epithelial expression from NAT tissues towards the tumor. SFRP1 was unmethylated in laser microdissected myofibroblasts and partially hypermethylated in epithelial cells in these areas. In contrast, we found epigenetically silenced myofibroblast-derived SFRP1 in CRC stroma. Our results suggest that the myofibroblast-derived SFRP1 protein might be a paracrine inhibitor of epithelial proliferation in NAT areas and loss of this signal may support tumor proliferation in CRC.

引用

页数：8

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