Mutational Analysis of the Cysteine-Rich Region of the Iron-Responsive GATA Factor Fep1. Role of Individual Cysteines as [2Fe-2S] Cluster Ligands

被引：7

作者：

di Patti, Maria Carmela Bonaccorsi ^{[1
]}

Cutone, Antimo ^{[2
]}

Musci, Giovanni ^{[2
]}

机构：

[1] Sapienza Univ Rome, Dept Biochem Sci, Rome, Italy

[2] Univ Molise, Dept Biosci & Terr, Pesche, Italy

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2018年 / 76卷 / 03期

关键词：

Iron; Iron-sulfur; Yeast; Fep1; PROTEIN SECONDARY STRUCTURE; FE-S CLUSTER; CIRCULAR-DICHROISM; PICHIA-PASTORIS; SULFUR PROTEINS; ZINC FINGERS; DNA-BINDING; COMPLEXES;

D O I：

10.1007/s12013-018-0842-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fep1, the iron-dependent GATA-type transcriptional repressor of the methylotrophic yeast Pichia pastoris, has a dimeric structure and binds an iron-sulfur cluster of the [2Fe-2S] type. In this work, we extend the characterization of this protein by analysis of the optical and CD spectroscopic properties of a set of mutants where cysteines within the conserved Cys-X-5-Cys-X(8-)Cys-X-2-Cys motif have been targeted, in order to evaluate their role as [2Fe-2S] ligands. The results suggest that all four cysteine residues are essential because replacing them with serines in different combinations invariably produces a protein unable to correctly bind the [2Fe-2S] cluster.

引用

页码：339 / 344

页数：6

共 3 条

[1] Mutational Analysis of the Cysteine-Rich Region of the Iron-Responsive GATA Factor Fep1. Role of Individual Cysteines as [2Fe–2S] Cluster Ligands
Maria Carmela Bonaccorsi di Patti
Antimo Cutone
Giovanni Musci
Cell Biochemistry and Biophysics, 2018, 76 : 339 - 344
[2] Peptide Mimics of the Cysteine-Rich Regions of HapX and SreA Bind a [2Fe-2S] Cluster In Vitro
Rossetto, Daniele
Sebastianelli, Lorenzo
Oberegger, Simon
Todorovic, Smilja
Haas, Hubertus
Mansy, Sheref S.
ADVANCED BIOLOGY, 2024, 8 (05):
[3] Pichia pastoris Fep1 is a [2Fe-2S] protein with a Zn finger that displays an unusual oxygen-dependent role in cluster binding
Cutone, Antimo
Howes, Barry D.
Miele, Adriana E.
Miele, Rossella
Giorgi, Alessandra
Battistoni, Andrea
Smulevich, Giulietta
Musci, Giovanni
di Patti, Maria Carmela Bonaccorsi
SCIENTIFIC REPORTS, 2016, 6

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