A genetic approach to study polyubiquitination in Saccharomyces cerevisiae

被引：2

作者：

Meza-Gutierrez, Fernando ^{[1
]}

Simsek, Deniz ^{[2
]}

Toczyski, David Paul ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Biochem, Helen Diller Family Comprehens Canc Ctr, San Francisco, CA 94143 USA

[2] Amgen Inc, San Francisco, CA USA

来源：

UBIQUITIN AND UBIQUITIN-LIKE PROTEIN MODIFIERS | 2019年 / 618卷

关键词：

UBIQUITIN CHAINS; DNA-REPAIR; YEAST; PARKIN; COMPLEX; ORGANIZATION; RESOLUTION; REVEAL; KINASE; MUTANT;

D O I：

10.1016/bs.mie.2018.12.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitin has seven lysines, all of which are used to generate polyubiquitin chains in the yeast Saccharomyces cerevisiae. While the biology associated with chains formed through lysines 48 and 63 is well studied, other chain types are more poorly characterized. We outline a methodology for using synthetic genetic analysis to examine ubiquitin mutants. Ubiquitin is encoded by four loci, necessitating several alterations to standard protocols, including the use of the SK1 strain background, which sporulates with very high efficiency. The methods described here could be used to examine other ubiquitin mutants, including those that do not support viability.

引用

页码：49 / 72

页数：24

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