Serotonin (5-HT) and 5-HT2A receptor agonists suppress lipolysis in primary rat adipose cells

被引：25

作者：

Hansson, Bjorn ^{[1
]}

Medina, Anya ^{[2
]}

Fryklund, Claes ^{[1
]}

Fex, Malin ^{[2
]}

Stenkula, Karin G. ^{[1
]}

机构：

[1] Lund Univ, Ctr Diabet, Ctr Biomed, Dept Expt Med Sci,Glucose Transport & Prot Traffi, S-22184 Lund, Sweden

[2] Lund Univ, Ctr Diabet, Clin Res Ctr, Dept Clin Sci,Unit Mol Metab, S-20502 Malmo, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 474卷 / 02期

基金：

瑞典研究理事会;

关键词：

Serotonin; 5-HT; Adipose cells; Lipid metabolism; Lipolysis; PERIPHERAL SEROTONIN; INSULIN-RESISTANCE; GLUCOSE; 5-HYDROXYTRYPTAMINE; ADIPOCYTES; ACTIVATION; EXPRESSION; SECRETION; TRANSPORT; CALCIUM;

D O I：

10.1016/j.bbrc.2016.04.110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5 HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed beta-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PICA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and isobuthylmethylxanthine (IBMX). The relative expression of 5-HT1A,-2B and-4 receptor class family were significantly higher in adipose tissue compared to adipose cells, whereas 5-HT1D,-2A and -7 were highly expressed in isolated adipose cells. Similar to 5-HT, 5-HT2 receptor agonists reduced lipolysis while 5-HT1 receptor agonists rather decreased non-stimulated and insulin-stimulated glucose uptake. Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：357 / 363

页数：7

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