Cytochrome c′ from Chromatium vinosum on gold electrodes

被引：18
作者：
Ge, B
Meyer, T
Schöning, MJ
Wollenberger, U
Lisdat, F
机构：
[1] Univ Potsdam, Inst Biochem & Mol Physiol, D-14476 Golm, Germany
[2] Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA
[3] Forschungszentrum Julich, Res Ctr, Inst Thin Film & Ion Technol, D-52425 Julich, Germany
[4] Univ Appl Sci Aachen, Aachen, Germany
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2000年 / 2卷 / 08期
基金：
美国国家卫生研究院;
关键词：
cytochrome c '; Chromatium vinosum; nitric oxide; modified gold electrode;
D O I：
10.1016/S1388-2481(00)00082-5
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Cytochrome c' from Chromatium vinosum (CVCP) was immobilized at a surface-modified gold electrode. Characterization of the CVCP electrode showed a quasi-reversible, diffusionless electrochemical redox behavior of the surface adsorbed protein with a formal potential of - 128 +/- 5 mV vs. Ag/AgCl. The heterogeneous electron transfer rate constant of adsorbed CVCP was determined to be about 50 s(-1). Different immobilization strategies were compared. The interaction of the immobilized CVCP with nitric oxide (NO) was investigated and applied for a primary amperometric detection of NO in solution. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码：557 / 561
页数：5
相关论文
共 24 条
[1]   PH VARIATION OF MIDPOINT POTENTIAL FOR 3 PHOTOSYNTHETIC BACTERIAL CYTOCHROMES-C' - A LINK BETWEEN PHYSICAL AND FUNCTIONAL-PROPERTIES [J].
BARAKAT, R ;
STREKAS, TC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 679 (03) :393-399
[2]   Fiber-optic nitric oxide-selective biosensors and nanosensors [J].
Barker, SLR ;
Kopelman, R ;
Meyer, TE ;
Cusanovich, MA .
ANALYTICAL CHEMISTRY, 1998, 70 (05) :971-976
[3]   Ratiometric and fluorescence lifetime-based biosensors incorporating cytochrome c′ and the detection of extra- and intracellular macrophage nitric oxide [J].
Barker, SLR ;
Clark, HA ;
Swallen, SF ;
Kopelman, R ;
Tsang, AW ;
Swanson, JA .
ANALYTICAL CHEMISTRY, 1999, 71 (09) :1767-1772
[4]  
BARTSCH RG, 1971, J BIOL CHEM, V246, P4489
[5]   NMR ASSIGNMENT OF RHODOBACTER-CAPSULATUS FERRICYTOCHROME C', A 28-KDA PARAMAGNETIC HEME PROTEIN [J].
CAFFREY, M ;
SIMORRE, JP ;
BRUTSCHER, B ;
CUSANOVICH, M ;
MARION, D .
BIOCHEMISTRY, 1995, 34 (17) :5904-5912
[6]   DIRECT ELECTRON-TRANSFER REACTIONS BETWEEN IMMOBILIZED CYTOCHROME-C AND MODIFIED GOLD ELECTRODES [J].
COOPER, JM ;
GREENOUGH, KR ;
MCNEIL, CJ .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1993, 347 (1-2) :267-275
[7]   LIGAND-CONTROLLED DISSOCIATION OF CHROMATIUM-VINOSUM CYTOCHROME-C' [J].
DOYLE, ML ;
GILL, SJ ;
CUSANOVICH, MA .
BIOCHEMISTRY, 1986, 25 (09) :2509-2516
[8]   DIRECT VOLTAMMETRIC BEHAVIOR OF RS-RUBRUM CYTOCHROME-C' AT A 2-MERCAPTOSUCCINATE-MODIFIED GOLD ELECTRODE [J].
ERABI, T ;
OZAWA, S ;
HAYASE, S ;
WADA, M .
CHEMISTRY LETTERS, 1992, (11) :2115-2118
[9]   BINDING OF CYANIDE TO CYTOCHROME-C' FROM CHROMATIUM-VINOSUM [J].
KASSNER, RJ ;
KYKTA, MG ;
CUSANOVICH, MA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 831 (01) :155-158
[10]   GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS [J].
LAVIRON, E .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) :19-28
123