Plant chitinase as a possible biocontrol agent for use instead of chemical fungicides

被引:81
作者
Karasuda, S
Tanaka, S
Kajihara, H
Yamamoto, Y
Koga, D [1 ]
机构
[1] Yamaguchi Univ, Fac Agr, Yamaguchi 7538515, Japan
[2] Yamaguchi Agr Expt Stn, Yamaguchi 7530214, Japan
关键词
biocontrol agent; class IV chitinase; family; 19; chitinase; powdery mildew; yam;
D O I
10.1271/bbb.67.221
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We investigated whether a plant chitinase can be used as a biocontrol agent instead of chemical fungicides by spraying chitinase E (family 19; class IV) from a yam (Dioscorea opposita Thunb) alone or together with beta-1,3-glucanase directly onto the surface of a powdery mildew infecting strawberry berries and leaves. Results were observed by eye and with a scanning electron microscope. The powdery mildew infecting the strawberries was degraded, mainly by the chitinase, and the disease did not appear again for more than 2 weeks. These results indicated that this kind of plant chitinase might be safe and biodegradable biocontrol agent for use instead of conventional fungicides.
引用
收藏
页码:221 / 224
页数:4
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