Application of DNA barcoding to the entire traditional Chinese medicine industrial chain: A case study of Rhei Radix et Rhizoma

被引：10

作者：

Xin, Tianyi ^{[1
]}

Li, Ranjun ^{[1
,2
]}

Lou, Qian ^{[1
]}

Lin, Yulin ^{[1
]}

Liao, Hai ^{[2
]}

Sun, Wei ^{[3
]}

Guan, Meng ^{[4
]}

Zhou, Jiayu ^{[2
]}

Song, Jingyuan ^{[1
,5
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Engn Res Ctr Chinese Med Resource, Key Lab Chinese Med Resources Conservat,Minist Edu, Beijing 100193, Peoples R China

[2] Southwest Jiaotong Univ, Sch Life & Sci, Chengdu 610031, Peoples R China

[3] China Acad Chinese Med Sci, Inst Chinese Mat Med, Key Lab Beijing Identificat & Safety Evaluat Chine, Beijing 100070, Peoples R China

[4] Sinopharm Tradit Chinese Med Co Ltd, Beijing 100097, Peoples R China

[5] Chinese Acad Med Sci, Yunnan Branch Inst Med Plant Dev, Yunnan Key Lab Southern Med Utilizat, Beijing 666100, Peoples R China

来源：

PHYTOMEDICINE | 2022年 / 105卷

基金：

中国国家自然科学基金;

关键词：

DNA barcoding; Entire industrial chain; Rhei Radix et Rhizoma; ndhF-rpl32; Species identification; AUTHENTICATION; IDENTIFICATION;

D O I：

10.1016/j.phymed.2022.154375

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Safety concerns, caused by complex and unpredictable adulterants, run through the entire industrial chain of traditional Chinese medicines (TCMs). However, the conventional circulation traceability system only focuses on a certain end or link at the back end of the TCM industrial chain, ignoring the integrity of the links cross the entire industrial chain and lacking traceability. In consequence, a strict and rational supervision system is urgently required for the entire industrial chain. Hypothesis/Purpose: We hypothesize that DNA barcoding would be a suitable measure for the traceability of adulterants in the entire TCM industrial chain. Methods: In this study, Rhei Radix et Rhizoma was selected as a model to establish a traceability system for the entire TCM industrial chain. A total of 110 samples, including leaves, seeds, roots, decoction pieces, and traditional Chinese patent medicines (TCPMs), were collected upstream, midstream, and downstream of the entire industrial chain of Rhei Radix et Rhizoma. The ndhF-rpl32 fragment rather than the universal DNA barcodes, which could not distinguish the three original species of Rhei Radix et Rhizoma, was selected as a specific DNA barcode to evaluate the practical application of DNA barcoding in the chain. Results: The results showed that the ndhF-rpl32 fragment in all samples could be amplified and bi-directionally sequenced. Based on the standard operating procedures of DNA barcoding, the ndhF-rpl32 fragment clearly distinguished the seven Rheum species collected upstream of the entire industrial chain. For the samples collected midstream and downstream of the entire industrial chain, 25% of the 36 commercial decoction pieces samples were identified as adulterants, whereas the eight TCPM samples were all derived from genuine Rhei Radix et Rhizoma. Conclusions: This study shows that DNA barcoding is a powerful and suitable technology that can be applied to trace TCMs in the entire industrial chain, thereby assuring clinical medication safety.

引用

页数：8